BBa_K175035
type: ComponentDefinition
persistent identity: BBa K175035
display id: BBa_K175035
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive expression of GFP with medium RBS lock and inducible production of key for the lock
created: 2009-09-30T11:00:00Z
modified: 2015-05-08T01:10:59Z
mutable provenance: This biobrick was assembled with biobricks from the registry and two new biobricks designed and constructed by TUDelft iGEM 09.
owned by: james
owned by: myers
mutable description: This biobrick has been used to test the functionality of the Lock/key for medium RBS (B0032).
discontinued: false
dominant: false
group u list: _280_
m user id: 0
owner id: 4299
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: ---
star: false
creator: Daniel Solis Escalante
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_B0010
component: BBa_E0040
component: BBa_K175031
component: BBa_R0040
component: BBa_K175032
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_K175032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_K175031
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K175035_sequence/1

BBa_B0012
type: Component
persistent identity: component2027827
display id: component2027827
version: 1
top level: BBa_K175035
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2027825
display id: component2027825
version: 1
top level: BBa_K175035
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component2027824
display id: component2027824
version: 1
top level: BBa_K175035
definition: GFP
access: public

BBa_K175031
type: Component
persistent identity: component2027822
display id: component2027822
version: 1
top level: BBa_K175035
definition: LMR
access: public

BBa_R0040
type: Component
persistent identity: component2027816
display id: component2027816
version: 1
top level: BBa_K175035
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component2027812
display id: component2027812
version: 1
top level: BBa_K175035
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2027810
display id: component2027810
version: 1
top level: BBa_K175035
definition: BBa_B0010
access: public

BBa_K175032
type: Component
persistent identity: component2027809
display id: component2027809
version: 1
top level: BBa_K175035
definition: KMR
access: public

BBa_R0010
type: Component
persistent identity: component2027802
display id: component2027802
version: 1
top level: BBa_K175035
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2027802
display id: annotation2027802
version: 1
top level: BBa_K175035
location: https://synbiohub.org/public/igem/BBa_K175035/annotation2027802/range2027802/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K175035/annotation2027802/range2027802/1
type: Range
persistent identity: range2027802
display id: range2027802
version: 1
top level: BBa_K175035
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_K175032
type: SequenceAnnotation
persistent identity: annotation2027809
display id: annotation2027809
version: 1
top level: BBa_K175035
location: https://synbiohub.org/public/igem/BBa_K175035/annotation2027809/range2027809/1
component: BBa_K175032

https://synbiohub.org/public/igem/BBa_K175035/annotation2027809/range2027809/1
type: Range
persistent identity: range2027809
display id: range2027809
version: 1
top level: BBa_K175035
direction: direction/reverse
start: 209
end: 292
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2027810
display id: annotation2027810
version: 1
top level: BBa_K175035
location: https://synbiohub.org/public/igem/BBa_K175035/annotation2027810/range2027810/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K175035/annotation2027810/range2027810/1
type: Range
persistent identity: range2027810
display id: range2027810
version: 1
top level: BBa_K175035
direction: direction/forward
start: 301
end: 380
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2027812
display id: annotation2027812
version: 1
top level: BBa_K175035
location: https://synbiohub.org/public/igem/BBa_K175035/annotation2027812/range2027812/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K175035/annotation2027812/range2027812/1
type: Range
persistent identity: range2027812
display id: range2027812
version: 1
top level: BBa_K175035
direction: direction/forward
start: 389
end: 429
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2027816
display id: annotation2027816
version: 1
top level: BBa_K175035
location: https://synbiohub.org/public/igem/BBa_K175035/annotation2027816/range2027816/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K175035/annotation2027816/range2027816/1
type: Range
persistent identity: range2027816
display id: range2027816
version: 1
top level: BBa_K175035
direction: direction/forward
start: 438
end: 491
orientation: inline

BBa_K175031
type: SequenceAnnotation
persistent identity: annotation2027822
display id: annotation2027822
version: 1
top level: BBa_K175035
location: https://synbiohub.org/public/igem/BBa_K175035/annotation2027822/range2027822/1
component: BBa_K175031

https://synbiohub.org/public/igem/BBa_K175035/annotation2027822/range2027822/1
type: Range
persistent identity: range2027822
display id: range2027822
version: 1
top level: BBa_K175035
direction: direction/reverse
start: 500
end: 546
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2027824
display id: annotation2027824
version: 1
top level: BBa_K175035
location: https://synbiohub.org/public/igem/BBa_K175035/annotation2027824/range2027824/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K175035/annotation2027824/range2027824/1
type: Range
persistent identity: range2027824
display id: range2027824
version: 1
top level: BBa_K175035
direction: direction/reverse
start: 553
end: 1272
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2027825
display id: annotation2027825
version: 1
top level: BBa_K175035
location: https://synbiohub.org/public/igem/BBa_K175035/annotation2027825/range2027825/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K175035/annotation2027825/range2027825/1
type: Range
persistent identity: range2027825
display id: range2027825
version: 1
top level: BBa_K175035
direction: direction/forward
start: 1281
end: 1360
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2027827
display id: annotation2027827
version: 1
top level: BBa_K175035
location: https://synbiohub.org/public/igem/BBa_K175035/annotation2027827/range2027827/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K175035/annotation2027827/range2027827/1
type: Range
persistent identity: range2027827
display id: range2027827
version: 1
top level: BBa_K175035
direction: direction/forward
start: 1369
end: 1409
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

LMR
type: ComponentDefinition
persistent identity: BBa K175031
display id: BBa_K175031
version: 1
was generated by: iGEM to SBOL conversion
description: Lock for medium RBS (B0032) from the lock/key library TUD09
created: 2009-09-30T11:00:00Z
modified: 2015-05-08T01:10:59Z
mutable provenance: The biobrick was designed using the lock/key library algorithm constructed by TUDelft iGEM 09 team. The algorithm is based on the work of Isaacs F. et al 2004, Berkeley iGEM 2006, Caltech iGEM 2007 and Peking iGEM 2007.
owned by: james
owned by: myers
mutable description: This biobrick generates a mRNA which forms a secondary structure blocking the (medium) RBS.
discontinued: false
dominant: false
experience: experience/None
group u list: _280_
m user id: 0
owner id: 4299
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Functionality is being tested
star: false
creator: Daniel Solis Escalante
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence annotation: Medium RBS B0032
sequence: https://synbiohub.org/public/igem/BBa_K175031_sequence/1

Medium RBS B0032
type: SequenceAnnotation
persistent identity: annotation2027755
display id: annotation2027755
version: 1
top level: LMR
location: https://synbiohub.org/public/igem/BBa_K175031/annotation2027755/range2027755/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K175031/annotation2027755/range2027755/1
type: Range
persistent identity: range2027755
display id: range2027755
version: 1
top level: LMR
direction: direction/forward
start: 35
end: 47
orientation: inline

KMR
type: ComponentDefinition
persistent identity: BBa K175032
display id: BBa_K175032
version: 1
was generated by: iGEM to SBOL conversion
description: Key for lock of medium (K175031) RBS (B0032) from the lock/key library TUD09
created: 2009-09-30T11:00:00Z
modified: 2015-05-08T01:10:59Z
mutable provenance: The biobrick was designed using the lock/key library algorithm constructed by TUDelft iGEM 09 team. The algorithm is based on the work of Isaacs F. et al 2004, Berkeley iGEM 2006, Caltech iGEM 2007 and Peking iGEM 2007.
owned by: james
owned by: myers
mutable description: This biobrick generates a mRNA which forms a secondary structure that opens LMR (K175031).
discontinued: false
dominant: false
experience: experience/None
group u list: _280_
m user id: 0
owner id: 4299
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Functionality is being tested
star: false
creator: Daniel Solis Escalante
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K175032_sequence/1

https://synbiohub.org/public/igem/BBa_K175035_sequence/1
type: Sequence
persistent identity: BBa K175035 sequence
display id: BBa_K175035_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagacccaaagtcctcacacaggaaagtggttaatgaaaattaacttactttcctgactgaaaaaagccgagttattaatccggctttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagaggtactatcctgtgtgaggactttgggtagatcactcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K175031_sequence/1
type: Sequence
persistent identity: BBa K175031 sequence
display id: BBa_K175031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtactatcctgtgtgaggactttgggtagatcactcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K175032_sequence/1
type: Sequence
persistent identity: BBa K175032 sequence
display id: BBa_K175032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acccaaagtcctcacacaggaaagtggttaatgaaaattaacttactttcctgactgaaaaaagccgagttattaatccggctt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z