LOCUS       BBa_K175039              921 bp    DNA     linear       07-Dec-2025
DEFINITION  I-SceI restriction site plus TetR generator
ACCESSION   BBa_K175039
VERSION     BBa_K175039.1  
FEATURES             Location/Qualifiers
     misc_feature    1..30
                     /label=I-SceI homing endonuclease restriction site
     misc_feature    1..30
                     /label=BBa_K175027
     promoter        39..73
                     /label=BBa_J23116
     misc_feature    86..89
                     /label=conserved
     RBS             82..93
                     /label=BBa_B0034
     CDS             103..719
                     /label=tetR
     misc_feature    720..753
                     /label=SsrA
     misc_feature    760..784
                     /label=Help:Barcodes
     CDS             100..784
                     /label=BBa_C0040
     stem_loop       804..847
                     /label=stem_loop
     misc_feature    793..872
                     /label=BBa_B0010
     terminator      793..872
                     /label=BBa_B0010
     stem_loop       888..907
                     /label=T7 TE
     misc_feature    914..914
                     /label=stop
     polyA_site      908..921
                     /label=polya
     misc_feature    881..921
                     /label=BBa_B0012
     terminator      881..921
                     /label=BBa_B0012
     terminator      793..921
                     /label=BBa_B0015
     misc_feature    39..921
                     /label=BBa_K145201
ORIGIN
        1 agttacgcta gggataacag ggtaatatag tactagagtt gacagctagc tcagtcctag
       61 ggactatgct agctactaga gaaagaggag aaatactaga tgtccagatt agataaaagt
      121 aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc
      181 cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat
      241 aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt
      301 tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga
      361 tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa
      421 aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta
      481 gagaatgcat tatatgcact cagcgctgtg gggcatttta ctttaggttg cgtattggaa
      541 gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg
      601 ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta
      661 ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc
      721 gctgcaaacg acgaaaacta cgctttagta gcttaataac actgatagtg ctagtgtaga
      781 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      841 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      901 gtgggccttt ctgcgtttat a
//