##gff-version 3
##sequence-region BBa_K175039 1 921
BBa_K175039	.	engineered_region	39	921	.	+	0	ID=BBa_K145201;Name=BBa_K145201
BBa_K175039	.	terminator	793	921	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K145201
BBa_K175039	.	terminator	793	872	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K175039	.	engineered_region	793	872	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K175039	.	stem_loop	804	847	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K175039	.	terminator	881	921	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K175039	.	engineered_region	881	921	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K175039	.	polyA_site	908	921	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K175039	.	stop_codon	914	914	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K175039	.	stem_loop	888	907	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K175039	.	ribosome_entry_site	82	93	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K145201
BBa_K175039	.	conserved_region	86	89	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K175039	.	CDS	100	784	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K145201
BBa_K175039	.	engineered_tag	760	784	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K175039	.	CDS	103	719	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K175039	.	tag	720	753	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K175039	.	promoter	39	73	.	+	0	ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_K145201
BBa_K175039	.	sequence_feature	1	30	.	+	0	ID=BBa_K175027;Name=BBa_K175027
BBa_K175039	.	sequence_feature	1	30	.	+	0	ID=annotation2014585;Name=I-SceI homing endonuclease restriction site;Parent=BBa_K175027
>BBa_K175039
agttacgctagggataacagggtaatatagtactagagttgacagctagctcagtcctagggactatgctagctactag
agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggt
cggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaa
aataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaagggg
aaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagt
acatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttt
tcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatc
aagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttga
tcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaa
agtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata