LOCUS       BBa_K175040             1214 bp    DNA     linear       22-Dec-2024
DEFINITION  I-SceI-LVA under p(LacI) + p(tetR) + I-SceI RS
ACCESSION   BBa_K175040
VERSION     BBa_K175040.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     CDS             230..977
                     /label=SceI
     CDS             230..977
                     /label=BBa_K142200
     stem_loop       997..1040
                     /label=stem_loop
     misc_feature    986..1065
                     /label=BBa_B0010
     terminator      986..1065
                     /label=BBa_B0010
     stem_loop       1081..1100
                     /label=T7 TE
     misc_feature    1107..1107
                     /label=stop
     polyA_site      1101..1114
                     /label=polya
     misc_feature    1074..1114
                     /label=BBa_B0012
     terminator      1074..1114
                     /label=BBa_B0012
     misc_feature    1123..1141
                     /label=TetR 1
     misc_feature    1123..1176
                     /label=BBa_R0040
     promoter        1142..1147
                     /label=-35
     misc_feature    1148..1166
                     /label=TetR 2
     promoter        1165..1170
                     /label=-10
     promoter        1123..1176
                     /label=BBa_R0040
     misc_feature    1185..1214
                     /label=I-SceI homing endonuclease restriction site
     misc_feature    1185..1214
                     /label=BBa_K175027
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgcatcaaaa
      241 aaaccaggta atgaacctgg gtccgaactc taaactgctg aaagaataca aatcccagct
      301 gatcgaactg aacatcgaac agttcgaagc aggtatcggt ctgatcctgg gtgatgctta
      361 catccgttct cgtgatgaag gtaaaaccta ctgtatgcag ttcgagtgga aaaacaaagc
      421 atacatggac cacgtatgtc tgctgtacga tcagtgggta ctgtccccgc cgcacaaaaa
      481 agaacgtgtt aaccacctgg gtaacctggt aatcacctgg ggcgcccaga ctttcaaaca
      541 ccaagccttc aacaaactgg ctaacctgtt catcgttaac aacaaaaaaa ccatcccgaa
      601 caacctggtt gaaaactacc tgaccccgat gtctctggca tactggttca tggatgatgg
      661 tggtaaatgg gattacaaca aaaactctac caacaaatcg atcgtactga acacccagtc
      721 tttcactttc gaagaagtag aatacctggt taagggtctg cgtaacaaat tccaactgaa
      781 ctgttacgta aaaatcaaca aaaacaaacc gatcatctac atcgattcta tgtcttacct
      841 gatcttctac aacctgatca aaccgtacct gatcccgcag atgatgtaca aactgccgaa
      901 cactatctcc tccgaaactt tccttaagag gcccgctgca aacgacgaaa actacgcttt
      961 agtagcttaa gtaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     1021 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     1081 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agtccctatc agtgatagag
     1141 attgacatcc ctatcagtga tagagatact gagcactact agagagttac gctagggata
     1201 acagggtaat atag
//