LOCUS       BBa_K175042             1844 bp    DNA     linear       19-Dec-2024
DEFINITION  GFP-LVA translational unit + I-SceI RS + TetR generator
ACCESSION   BBa_K175042
VERSION     BBa_K175042.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     misc_feature    20..22
                     /label=start
     misc_feature    773..775
                     /label=stop
     misc_feature    776..778
                     /label=stop
     misc_feature    736..772
                     /label=LVA tag
     CDS             23..735
                     /label=cds
     misc_feature    20..778
                     /label=BBa_K145015
     stem_loop       798..841
                     /label=stem_loop
     misc_feature    787..866
                     /label=BBa_B0010
     terminator      787..866
                     /label=BBa_B0010
     stem_loop       882..901
                     /label=T7 TE
     misc_feature    908..908
                     /label=stop
     polyA_site      902..915
                     /label=polya
     misc_feature    875..915
                     /label=BBa_B0012
     terminator      875..915
                     /label=BBa_B0012
     misc_feature    1..915
                     /label=BBa_K175038
     misc_feature    924..953
                     /label=I-SceI homing endonuclease restriction site
     misc_feature    924..953
                     /label=BBa_K175027
     promoter        962..996
                     /label=BBa_J23116
     misc_feature    1009..1012
                     /label=conserved
     RBS             1005..1016
                     /label=BBa_B0034
     CDS             1026..1642
                     /label=tetR
     misc_feature    1643..1676
                     /label=SsrA
     misc_feature    1683..1707
                     /label=Help:Barcodes
     CDS             1023..1707
                     /label=BBa_C0040
     stem_loop       1727..1770
                     /label=stem_loop
     misc_feature    1716..1795
                     /label=BBa_B0010
     terminator      1716..1795
                     /label=BBa_B0010
     stem_loop       1811..1830
                     /label=T7 TE
     misc_feature    1837..1837
                     /label=stop
     polyA_site      1831..1844
                     /label=polya
     misc_feature    1804..1844
                     /label=BBa_B0012
     terminator      1804..1844
                     /label=BBa_B0012
     terminator      1716..1844
                     /label=BBa_B0015
     misc_feature    962..1844
                     /label=BBa_K145201
     misc_feature    924..1844
                     /label=BBa_K175039
ORIGIN
        1 tcacacagga aagtactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
       61 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      121 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      181 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
      241 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      301 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      361 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      421 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
      481 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
      541 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
      601 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
      661 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
      721 tgaactatac aaaaggcctg ctgcaaacga cgaaaactac gctttagtag cttaataata
      781 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
      841 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
      901 ctttctgcgt ttatatacta gagagttacg ctagggataa cagggtaata tagtactaga
      961 gttgacagct agctcagtcc tagggactat gctagctact agagaaagag gagaaatact
     1021 agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
     1081 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
     1141 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
     1201 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
     1261 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
     1321 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
     1381 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
     1441 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
     1501 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
     1561 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
     1621 aacttaaatg tgaaagtggg tccgctgcaa acgacgaaaa ctacgcttta gtagcttaat
     1681 aacactgata gtgctagtgt agatcactac tagagccagg catcaaataa aacgaaaggc
     1741 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1801 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
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