##gff-version 3
##sequence-region BBa_K175042 1 1844
BBa_K175042	.	engineered_region	924	1844	.	+	0	ID=BBa_K175039;Name=BBa_K175039
BBa_K175042	.	sequence_feature	924	953	.	+	0	ID=BBa_K175027;Name=BBa_K175027;Parent=BBa_K175039
BBa_K175042	.	sequence_feature	924	953	.	+	0	ID=annotation2014585;Name=I-SceI homing endonuclease restriction site;Parent=BBa_K175027
BBa_K175042	.	engineered_region	962	1844	.	+	0	ID=BBa_K145201;Name=BBa_K145201;Parent=BBa_K175039
BBa_K175042	.	CDS	1023	1707	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K145201
BBa_K175042	.	tag	1643	1676	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K175042	.	engineered_tag	1683	1707	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K175042	.	CDS	1026	1642	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K175042	.	promoter	962	996	.	+	0	ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_K145201
BBa_K175042	.	ribosome_entry_site	1005	1016	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K145201
BBa_K175042	.	conserved_region	1009	1012	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K175042	.	terminator	1716	1844	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K145201
BBa_K175042	.	terminator	1804	1844	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K175042	.	polyA_site	1831	1844	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K175042	.	engineered_region	1804	1844	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K175042	.	stem_loop	1811	1830	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K175042	.	stop_codon	1837	1837	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K175042	.	terminator	1716	1795	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K175042	.	stem_loop	1727	1770	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K175042	.	engineered_region	1716	1795	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K175042	.	engineered_region	1	915	.	+	0	ID=BBa_K175038;Name=BBa_K175038
BBa_K175042	.	engineered_region	20	778	.	+	0	ID=BBa_K145015;Name=BBa_K145015;Parent=BBa_K175038
BBa_K175042	.	stop_codon	776	778	.	+	0	ID=annotation1969618;Name=stop;Parent=BBa_K145015
BBa_K175042	.	tag	736	772	.	+	0	ID=annotation1969619;Name=LVA tag;Parent=BBa_K145015
BBa_K175042	.	start_codon	20	22	.	+	0	ID=annotation1969616;Name=start;Parent=BBa_K145015
BBa_K175042	.	CDS	23	735	.	+	0	ID=annotation1969620;Name=cds;Parent=BBa_K145015
BBa_K175042	.	stop_codon	773	775	.	+	0	ID=annotation1969617;Name=stop;Parent=BBa_K145015
BBa_K175042	.	ribosome_entry_site	1	13	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K175038
BBa_K175042	.	engineered_region	1	13	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K175042	.	ribosome_entry_site	1	13	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K175042	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K175042	.	terminator	875	915	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K175038
BBa_K175042	.	polyA_site	902	915	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K175042	.	engineered_region	875	915	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K175042	.	stem_loop	882	901	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K175042	.	stop_codon	908	908	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K175042	.	terminator	787	866	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K175038
BBa_K175042	.	stem_loop	798	841	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K175042	.	engineered_region	787	866	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K175042
tcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggt
gatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaattta
tttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgag
atacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatattt
ttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaa
aaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacat
catggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaacta
gcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaat
ctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacaca
tggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagttacgctagggataacagggtaa
tatagtactagagttgacagctagctcagtcctagggactatgctagctactagagaaagaggagaaatactagatgtc
cagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgt
aaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgcct
tagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaa
taacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaa
aaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcac
tcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaac
acctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttc
ttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaa
actacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttata