LOCUS       BBa_K175044             1906 bp    DNA     linear       22-Dec-2024
DEFINITION  I-SceI restriction site + GFP-LVA under p(TetR)
ACCESSION   BBa_K175044
VERSION     BBa_K175044.1  
FEATURES             Location/Qualifiers
     misc_feature    1..30
                     /label=I-SceI homing endonuclease restriction site
     misc_feature    1..30
                     /label=BBa_K175027
     promoter        39..73
                     /label=BBa_J23116
     misc_feature    86..89
                     /label=conserved
     RBS             82..93
                     /label=BBa_B0034
     CDS             103..719
                     /label=tetR
     misc_feature    720..753
                     /label=SsrA
     misc_feature    760..784
                     /label=Help:Barcodes
     CDS             100..784
                     /label=BBa_C0040
     stem_loop       804..847
                     /label=stem_loop
     misc_feature    793..872
                     /label=BBa_B0010
     terminator      793..872
                     /label=BBa_B0010
     stem_loop       888..907
                     /label=T7 TE
     misc_feature    914..914
                     /label=stop
     polyA_site      908..921
                     /label=polya
     misc_feature    881..921
                     /label=BBa_B0012
     terminator      881..921
                     /label=BBa_B0012
     terminator      793..921
                     /label=BBa_B0015
     misc_feature    39..921
                     /label=BBa_K145201
     misc_feature    930..948
                     /label=TetR 1
     misc_feature    930..983
                     /label=BBa_R0040
     promoter        949..954
                     /label=-35
     misc_feature    955..973
                     /label=TetR 2
     promoter        972..977
                     /label=-10
     promoter        930..983
                     /label=BBa_R0040
     RBS             992..1004
                     /label=RBS-3\Weak
     RBS             998..1001
                     /label=RBS
     misc_feature    992..1004
                     /label=BBa_B0032
     RBS             992..1004
                     /label=BBa_B0032
     misc_feature    1011..1013
                     /label=start
     misc_feature    1764..1766
                     /label=stop
     misc_feature    1767..1769
                     /label=stop
     misc_feature    1727..1763
                     /label=LVA tag
     CDS             1014..1726
                     /label=cds
     misc_feature    1011..1769
                     /label=BBa_K145015
     stem_loop       1789..1832
                     /label=stem_loop
     misc_feature    1778..1857
                     /label=BBa_B0010
     terminator      1778..1857
                     /label=BBa_B0010
     stem_loop       1873..1892
                     /label=T7 TE
     misc_feature    1899..1899
                     /label=stop
     polyA_site      1893..1906
                     /label=polya
     misc_feature    1866..1906
                     /label=BBa_B0012
     terminator      1866..1906
                     /label=BBa_B0012
     misc_feature    930..1906
                     /label=BBa_K145205
     misc_feature    39..1906
                     /label=BBa_K145280
ORIGIN
        1 agttacgcta gggataacag ggtaatatag tactagagtt gacagctagc tcagtcctag
       61 ggactatgct agctactaga gaaagaggag aaatactaga tgtccagatt agataaaagt
      121 aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc
      181 cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat
      241 aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt
      301 tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga
      361 tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa
      421 aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta
      481 gagaatgcat tatatgcact cagcgctgtg gggcatttta ctttaggttg cgtattggaa
      541 gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg
      601 ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta
      661 ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc
      721 gctgcaaacg acgaaaacta cgctttagta gcttaataac actgatagtg ctagtgtaga
      781 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      841 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      901 gtgggccttt ctgcgtttat atactagagt ccctatcagt gatagagatt gacatcccta
      961 tcagtgatag agatactgag cactactaga gtcacacagg aaagtactag atgcgtaaag
     1021 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
     1081 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
     1141 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt
     1201 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt
     1261 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
     1321 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
     1381 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
     1441 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta
     1501 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
     1561 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
     1621 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
     1681 taacagctgc tgggattaca catggcatgg atgaactata caaaaggcct gctgcaaacg
     1741 acgaaaacta cgctttagta gcttaataat actagagcca ggcatcaaat aaaacgaaag
     1801 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     1861 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//