##gff-version 3
##sequence-region BBa_K175044 1 1906
BBa_K175044	.	sequence_feature	1	30	.	+	0	ID=BBa_K175027;Name=BBa_K175027
BBa_K175044	.	sequence_feature	1	30	.	+	0	ID=annotation2014585;Name=I-SceI homing endonuclease restriction site;Parent=BBa_K175027
BBa_K175044	.	engineered_region	39	1906	.	+	0	ID=BBa_K145280;Name=BBa_K145280
BBa_K175044	.	engineered_region	39	921	.	+	0	ID=BBa_K145201;Name=BBa_K145201;Parent=BBa_K145280
BBa_K175044	.	ribosome_entry_site	82	93	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K145201
BBa_K175044	.	conserved_region	86	89	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K175044	.	CDS	100	784	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K145201
BBa_K175044	.	engineered_tag	760	784	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K175044	.	tag	720	753	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K175044	.	CDS	103	719	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K175044	.	promoter	39	73	.	+	0	ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_K145201
BBa_K175044	.	terminator	793	921	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K145201
BBa_K175044	.	terminator	793	872	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K175044	.	engineered_region	793	872	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K175044	.	stem_loop	804	847	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K175044	.	terminator	881	921	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K175044	.	engineered_region	881	921	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K175044	.	stop_codon	914	914	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K175044	.	stem_loop	888	907	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K175044	.	polyA_site	908	921	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K175044	.	engineered_region	930	1906	.	+	0	ID=BBa_K145205;Name=BBa_K145205;Parent=BBa_K145280
BBa_K175044	.	terminator	1778	1857	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K145205
BBa_K175044	.	engineered_region	1778	1857	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K175044	.	stem_loop	1789	1832	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K175044	.	terminator	1866	1906	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K145205
BBa_K175044	.	engineered_region	1866	1906	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K175044	.	stop_codon	1899	1899	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K175044	.	stem_loop	1873	1892	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K175044	.	polyA_site	1893	1906	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K175044	.	promoter	930	983	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K145205
BBa_K175044	.	promoter	949	954	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K175044	.	non_covalent_binding_site	955	973	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K175044	.	engineered_region	930	983	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K175044	.	promoter	972	977	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K175044	.	non_covalent_binding_site	930	948	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K175044	.	ribosome_entry_site	992	1004	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K145205
BBa_K175044	.	ribosome_entry_site	998	1001	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K175044	.	ribosome_entry_site	992	1004	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K175044	.	engineered_region	992	1004	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K175044	.	engineered_region	1011	1769	.	+	0	ID=BBa_K145015;Name=BBa_K145015;Parent=BBa_K145205
BBa_K175044	.	stop_codon	1764	1766	.	+	0	ID=annotation1969617;Name=stop;Parent=BBa_K145015
BBa_K175044	.	start_codon	1011	1013	.	+	0	ID=annotation1969616;Name=start;Parent=BBa_K145015
BBa_K175044	.	CDS	1014	1726	.	+	0	ID=annotation1969620;Name=cds;Parent=BBa_K145015
BBa_K175044	.	stop_codon	1767	1769	.	+	0	ID=annotation1969618;Name=stop;Parent=BBa_K145015
BBa_K175044	.	tag	1727	1763	.	+	0	ID=annotation1969619;Name=LVA tag;Parent=BBa_K145015
>BBa_K175044
agttacgctagggataacagggtaatatagtactagagttgacagctagctcagtcctagggactatgctagctactag
agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggt
cggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaa
aataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaagggg
aaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagt
acatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttt
tcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatc
aagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttga
tcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaa
agtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagaga
ttgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtactagatgcgtaaaggagaaga
acttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagag
ggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaa
cacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaa
gagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaa
gtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttg
gacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaa
cttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgat
ggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagacc
acatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaa
cgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttata