LOCUS BBa_K175045 3028 bp DNA linear 05-Dec-2025 DEFINITION Self Destructive Plasmid with GFP-LVA reporter ACCESSION BBa_K175045 VERSION BBa_K175045.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..223 /label=RBS-1\Strong RBS 216..220 /label=RBS misc_feature 209..223 /label=BBa_B0030 RBS 209..223 /label=BBa_B0030 CDS 230..977 /label=SceI CDS 230..977 /label=BBa_K142200 stem_loop 997..1040 /label=stem_loop misc_feature 986..1065 /label=BBa_B0010 terminator 986..1065 /label=BBa_B0010 stem_loop 1081..1100 /label=T7 TE misc_feature 1107..1107 /label=stop polyA_site 1101..1114 /label=polya misc_feature 1074..1114 /label=BBa_B0012 terminator 1074..1114 /label=BBa_B0012 misc_feature 1..1114 /label=BBa_K175041 misc_feature 1123..1152 /label=I-SceI homing endonuclease restriction site misc_feature 1123..1152 /label=BBa_K175027 promoter 1161..1195 /label=BBa_J23116 misc_feature 1208..1211 /label=conserved RBS 1204..1215 /label=BBa_B0034 CDS 1225..1841 /label=tetR misc_feature 1842..1875 /label=SsrA misc_feature 1882..1906 /label=Help:Barcodes CDS 1222..1906 /label=BBa_C0040 stem_loop 1926..1969 /label=stem_loop misc_feature 1915..1994 /label=BBa_B0010 terminator 1915..1994 /label=BBa_B0010 stem_loop 2010..2029 /label=T7 TE misc_feature 2036..2036 /label=stop polyA_site 2030..2043 /label=polya misc_feature 2003..2043 /label=BBa_B0012 terminator 2003..2043 /label=BBa_B0012 terminator 1915..2043 /label=BBa_B0015 misc_feature 1161..2043 /label=BBa_K145201 misc_feature 2052..2070 /label=TetR 1 misc_feature 2052..2105 /label=BBa_R0040 promoter 2071..2076 /label=-35 misc_feature 2077..2095 /label=TetR 2 promoter 2094..2099 /label=-10 promoter 2052..2105 /label=BBa_R0040 RBS 2114..2126 /label=RBS-3\Weak RBS 2120..2123 /label=RBS misc_feature 2114..2126 /label=BBa_B0032 RBS 2114..2126 /label=BBa_B0032 misc_feature 2133..2135 /label=start misc_feature 2886..2888 /label=stop misc_feature 2889..2891 /label=stop misc_feature 2849..2885 /label=LVA tag CDS 2136..2848 /label=cds misc_feature 2133..2891 /label=BBa_K145015 stem_loop 2911..2954 /label=stem_loop misc_feature 2900..2979 /label=BBa_B0010 terminator 2900..2979 /label=BBa_B0010 stem_loop 2995..3014 /label=T7 TE misc_feature 3021..3021 /label=stop polyA_site 3015..3028 /label=polya misc_feature 2988..3028 /label=BBa_B0012 terminator 2988..3028 /label=BBa_B0012 misc_feature 2052..3028 /label=BBa_K145205 misc_feature 1161..3028 /label=BBa_K145280 misc_feature 1123..3028 /label=BBa_K175044 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgcatcaaaa 241 aaaccaggta atgaacctgg gtccgaactc taaactgctg aaagaataca aatcccagct 301 gatcgaactg aacatcgaac agttcgaagc aggtatcggt ctgatcctgg gtgatgctta 361 catccgttct cgtgatgaag gtaaaaccta ctgtatgcag ttcgagtgga aaaacaaagc 421 atacatggac cacgtatgtc tgctgtacga tcagtgggta ctgtccccgc cgcacaaaaa 481 agaacgtgtt aaccacctgg gtaacctggt aatcacctgg ggcgcccaga ctttcaaaca 541 ccaagccttc aacaaactgg ctaacctgtt catcgttaac aacaaaaaaa ccatcccgaa 601 caacctggtt gaaaactacc tgaccccgat gtctctggca tactggttca tggatgatgg 661 tggtaaatgg gattacaaca aaaactctac caacaaatcg atcgtactga acacccagtc 721 tttcactttc gaagaagtag aatacctggt taagggtctg cgtaacaaat tccaactgaa 781 ctgttacgta aaaatcaaca aaaacaaacc gatcatctac atcgattcta tgtcttacct 841 gatcttctac aacctgatca aaccgtacct gatcccgcag atgatgtaca aactgccgaa 901 cactatctcc tccgaaactt tccttaagag gcccgctgca aacgacgaaa actacgcttt 961 agtagcttaa gtaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 1021 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 1081 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agagttacgc tagggataac 1141 agggtaatat agtactagag ttgacagcta gctcagtcct agggactatg ctagctacta 1201 gagaaagagg agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca 1261 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag 1321 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac 1381 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa 1441 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat 1501 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc 1561 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca 1621 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc 1681 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct 1741 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc 1801 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac 1861 tacgctttag tagcttaata acactgatag tgctagtgta gatcactact agagccaggc 1921 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 1981 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 2041 atatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg 2101 agcactacta gagtcacaca ggaaagtact agatgcgtaa aggagaagaa cttttcactg 2161 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca 2221 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta 2281 ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat 2341 gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg 2401 aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg 2461 ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt 2521 ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac tcacacaatg 2581 tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca 2641 acattgaaga tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg 2701 atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag 2761 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta 2821 cacatggcat ggatgaacta tacaaaaggc ctgctgcaaa cgacgaaaac tacgctttag 2881 tagcttaata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 2941 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 3001 ccttcgggtg ggcctttctg cgtttata //