LOCUS       BBa_K175046             1724 bp    DNA     linear       06-May-2024
DEFINITION  Plasmid 1 for Transcriptional Cascade subproject TU Delft iGEM 09
ACCESSION   BBa_K175046
VERSION     BBa_K175046.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     promoter        1..74
                     /label=BBa_J13002
     CDS             84..791
                     /label=cI lambda
     misc_feature    792..824
                     /label=LVA
     misc_feature    831..855
                     /label=Help:Barcodes
     CDS             81..855
                     /label=BBa_C0051
     misc_feature    868..871
                     /label=conserved
     RBS             864..875
                     /label=BBa_B0034
     CDS             882..1556
                     /label=mrfp1
     misc_feature    1563..1587
                     /label=Help:Barcodes
     CDS             882..1562
                     /label=BBa_E1010
     stem_loop       1607..1650
                     /label=stem_loop
     misc_feature    1596..1675
                     /label=BBa_B0010
     terminator      1596..1675
                     /label=BBa_B0010
     stem_loop       1691..1710
                     /label=T7 TE
     misc_feature    1717..1717
                     /label=stop
     polyA_site      1711..1724
                     /label=polya
     misc_feature    1684..1724
                     /label=BBa_B0012
     terminator      1684..1724
                     /label=BBa_B0012
     misc_feature    864..1724
                     /label=BBa_I13507
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa gagcagcttg
      121 aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc
      181 aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct ttatttaatg
      241 gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc aaagttagcg
      301 ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa gcggttagta
      361 tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga
      421 tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa
      481 ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac
      541 caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg
      601 ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga
      661 aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga
      721 tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag
      781 agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt
      841 gctagtgtag atcgctacta gagaaagagg agaaatacta gatggcttcc tccgaagacg
      901 ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt
      961 tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga
     1021 aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt
     1081 acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct
     1141 tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg
     1201 ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta
     1261 ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca
     1321 ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac
     1381 tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg
     1441 ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag
     1501 actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat
     1561 aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc
     1621 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1681 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//