##gff-version 3
##sequence-region BBa_K175046 1 1724
BBa_K175046	.	promoter	1	74	.	+	0	ID=BBa_J13002;Name=BBa_J13002
BBa_K175046	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J13002
BBa_K175046	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K175046	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J13002
BBa_K175046	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K175046	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K175046	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K175046	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K175046	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K175046	.	CDS	81	855	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_K175046	.	CDS	84	791	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K175046	.	tag	792	824	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K175046	.	engineered_tag	831	855	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K175046	.	engineered_region	864	1724	.	+	0	ID=BBa_I13507;Name=BBa_I13507
BBa_K175046	.	CDS	882	1562	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K175046	.	CDS	882	1556	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K175046	.	engineered_tag	1563	1587	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K175046	.	ribosome_entry_site	864	875	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K175046	.	conserved_region	868	871	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K175046	.	terminator	1684	1724	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K175046	.	polyA_site	1711	1724	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K175046	.	stem_loop	1691	1710	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K175046	.	stop_codon	1717	1717	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K175046	.	engineered_region	1684	1724	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K175046	.	terminator	1596	1675	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K175046	.	stem_loop	1607	1650	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K175046	.	engineered_region	1596	1675	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K175046
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaag
aaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttattta
atggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagccc
ttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccct
gttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaa
gcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaa
gccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccaga
cttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagt
acccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttgg
cgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaagag
gagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgtta
acggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttac
caaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacac
ccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacg
gtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaa
cttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagac
ggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacct
acatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaaga
ctacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgt
agatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata