LOCUS       BBa_K175047             1988 bp    DNA     linear       29-Apr-2024
DEFINITION  Plasmid 2 for Transcriptional Cascade subproject TU Delft iGEM 09
ACCESSION   BBa_K175047
VERSION     BBa_K175047.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             230..846
                     /label=tetR
     misc_feature    847..880
                     /label=SsrA
     misc_feature    887..911
                     /label=Help:Barcodes
     CDS             227..886
                     /label=BBa_C0040
     stem_loop       931..974
                     /label=stem_loop
     misc_feature    920..999
                     /label=BBa_B0010
     terminator      920..999
                     /label=BBa_B0010
     stem_loop       1015..1034
                     /label=T7 TE
     misc_feature    1041..1041
                     /label=stop
     polyA_site      1035..1048
                     /label=polya
     misc_feature    1008..1048
                     /label=BBa_B0012
     terminator      1008..1048
                     /label=BBa_B0012
     misc_feature    209..1048
                     /label=BBa_P0440
     promoter        1094..1099
                     /label=-10
     promoter        1071..1076
                     /label=-35
     misc_feature    1081..1097
                     /label=OR1
     misc_feature    1057..1073
                     /label=OR2
     misc_feature    1057..1105
                     /label=BBa_R0051
     promoter        1057..1105
                     /label=BBa_R0051
     misc_feature    1118..1121
                     /label=conserved
     RBS             1114..1125
                     /label=BBa_B0034
     CDS             1132..1851
                     /label=GFP protein
     CDS             1132..1851
                     /label=BBa_E0040
     stem_loop       1871..1914
                     /label=stem_loop
     misc_feature    1860..1939
                     /label=BBa_B0010
     terminator      1860..1939
                     /label=BBa_B0010
     stem_loop       1955..1974
                     /label=T7 TE
     misc_feature    1981..1981
                     /label=stop
     polyA_site      1975..1988
                     /label=polya
     misc_feature    1948..1988
                     /label=BBa_B0012
     terminator      1948..1988
                     /label=BBa_B0012
     misc_feature    1114..1988
                     /label=BBa_I13504
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt ccagattaga
      241 taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt
      301 aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt
      361 aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac
      421 tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag
      481 ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc
      541 tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt
      601 ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt
      661 attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag
      721 tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc
      781 cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag
      841 tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta
      901 gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      961 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1021 ccttcgggtg ggcctttctg cgtttatata ctagagtaac accgtgcgtg ttgactattt
     1081 tacctctggc ggtgataatg gttgctacta gagaaagagg agaaatacta gatgcgtaaa
     1141 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
     1201 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
     1261 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact
     1321 ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt
     1381 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
     1441 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
     1501 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
     1561 aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt
     1621 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa
     1681 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca
     1741 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
     1801 gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata atactagagc
     1861 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1921 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1981 cgtttata
//