LOCUS       BBa_K175048             1084 bp    DNA     linear       22-Dec-2024
DEFINITION  GFP generator with medium RBS (B0032)
ACCESSION   BBa_K175048
VERSION     BBa_K175048.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..221
                     /label=RBS-3\Weak
     RBS             215..218
                     /label=RBS
     misc_feature    209..221
                     /label=BBa_B0032
     RBS             209..221
                     /label=BBa_B0032
     CDS             228..947
                     /label=GFP protein
     CDS             228..947
                     /label=BBa_E0040
     stem_loop       967..1010
                     /label=stem_loop
     misc_feature    956..1035
                     /label=BBa_B0010
     terminator      956..1035
                     /label=BBa_B0010
     stem_loop       1051..1070
                     /label=T7 TE
     misc_feature    1077..1077
                     /label=stop
     polyA_site      1071..1084
                     /label=polya
     misc_feature    1044..1084
                     /label=BBa_B0012
     terminator      1044..1084
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg cgtaaaggag
      241 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
      301 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
      361 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg
      421 gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca
      481 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
      541 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
      601 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
      661 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact
      721 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
      781 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
      841 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
      901 cagctgctgg gattacacat ggcatggatg aactatacaa ataataatac tagagccagg
      961 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1021 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1081 tata
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