LOCUS       BBa_K175050             1944 bp    DNA     linear       22-Dec-2024
DEFINITION  aTc inducible GFP-LVA generator with integrated I-SceI RS + I-SceI 
            RS + TetR generator
ACCESSION   BBa_K175050
VERSION     BBa_K175050.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..92
                     /label=I-SceI homing endonuclease restriction site
     misc_feature    63..92
                     /label=BBa_K175027
     misc_feature    1..92
                     /label=BBa_K175036
     RBS             101..113
                     /label=RBS-3\Weak
     RBS             107..110
                     /label=RBS
     misc_feature    101..113
                     /label=BBa_B0032
     RBS             101..113
                     /label=BBa_B0032
     misc_feature    120..122
                     /label=start
     misc_feature    873..875
                     /label=stop
     misc_feature    876..878
                     /label=stop
     misc_feature    836..872
                     /label=LVA tag
     CDS             123..835
                     /label=cds
     misc_feature    120..878
                     /label=BBa_K145015
     stem_loop       898..941
                     /label=stem_loop
     misc_feature    887..966
                     /label=BBa_B0010
     terminator      887..966
                     /label=BBa_B0010
     stem_loop       982..1001
                     /label=T7 TE
     misc_feature    1008..1008
                     /label=stop
     polyA_site      1002..1015
                     /label=polya
     misc_feature    975..1015
                     /label=BBa_B0012
     terminator      975..1015
                     /label=BBa_B0012
     misc_feature    101..1015
                     /label=BBa_K175038
     misc_feature    1024..1053
                     /label=I-SceI homing endonuclease restriction site
     misc_feature    1024..1053
                     /label=BBa_K175027
     promoter        1062..1096
                     /label=BBa_J23116
     misc_feature    1109..1112
                     /label=conserved
     RBS             1105..1116
                     /label=BBa_B0034
     CDS             1126..1742
                     /label=tetR
     misc_feature    1743..1776
                     /label=SsrA
     misc_feature    1783..1807
                     /label=Help:Barcodes
     CDS             1123..1807
                     /label=BBa_C0040
     stem_loop       1827..1870
                     /label=stem_loop
     misc_feature    1816..1895
                     /label=BBa_B0010
     terminator      1816..1895
                     /label=BBa_B0010
     stem_loop       1911..1930
                     /label=T7 TE
     misc_feature    1937..1937
                     /label=stop
     polyA_site      1931..1944
                     /label=polya
     misc_feature    1904..1944
                     /label=BBa_B0012
     terminator      1904..1944
                     /label=BBa_B0012
     terminator      1816..1944
                     /label=BBa_B0015
     misc_feature    1062..1944
                     /label=BBa_K145201
     misc_feature    1024..1944
                     /label=BBa_K175039
     misc_feature    101..1944
                     /label=BBa_K175042
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agagttacgc tagggataac agggtaatat agtactagag tcacacagga aagtactaga
      121 tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
      181 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa
      241 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg
      301 tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc
      361 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca
      421 aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta
      481 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat
      541 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa
      601 tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc
      661 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
      721 tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc
      781 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaaaggcctg
      841 ctgcaaacga cgaaaactac gctttagtag cttaataata ctagagccag gcatcaaata
      901 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
      961 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta
     1021 gagagttacg ctagggataa cagggtaata tagtactaga gttgacagct agctcagtcc
     1081 tagggactat gctagctact agagaaagag gagaaatact agatgtccag attagataaa
     1141 agtaaagtga ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca
     1201 acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa
     1261 aataagcggg ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac
     1321 ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt
     1381 agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca
     1441 gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca
     1501 ctagagaatg cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg
     1561 gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg
     1621 ccgccattat tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc
     1681 ttattcggcc ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg
     1741 tccgctgcaa acgacgaaaa ctacgcttta gtagcttaat aacactgata gtgctagtgt
     1801 agatcactac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1861 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1921 cgggtgggcc tttctgcgtt tata
//