##gff-version 3
##sequence-region BBa_K175050 1 1944
BBa_K175050	.	engineered_region	101	1944	.	+	0	ID=BBa_K175042;Name=BBa_K175042
BBa_K175050	.	engineered_region	101	1015	.	+	0	ID=BBa_K175038;Name=BBa_K175038;Parent=BBa_K175042
BBa_K175050	.	ribosome_entry_site	101	113	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K175038
BBa_K175050	.	ribosome_entry_site	107	110	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K175050	.	engineered_region	101	113	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K175050	.	ribosome_entry_site	101	113	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K175050	.	engineered_region	120	878	.	+	0	ID=BBa_K145015;Name=BBa_K145015;Parent=BBa_K175038
BBa_K175050	.	tag	836	872	.	+	0	ID=annotation1969619;Name=LVA tag;Parent=BBa_K145015
BBa_K175050	.	stop_codon	873	875	.	+	0	ID=annotation1969617;Name=stop;Parent=BBa_K145015
BBa_K175050	.	stop_codon	876	878	.	+	0	ID=annotation1969618;Name=stop;Parent=BBa_K145015
BBa_K175050	.	start_codon	120	122	.	+	0	ID=annotation1969616;Name=start;Parent=BBa_K145015
BBa_K175050	.	CDS	123	835	.	+	0	ID=annotation1969620;Name=cds;Parent=BBa_K145015
BBa_K175050	.	terminator	887	966	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K175038
BBa_K175050	.	stem_loop	898	941	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K175050	.	engineered_region	887	966	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K175050	.	terminator	975	1015	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K175038
BBa_K175050	.	engineered_region	975	1015	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K175050	.	stop_codon	1008	1008	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K175050	.	stem_loop	982	1001	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K175050	.	polyA_site	1002	1015	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K175050	.	engineered_region	1024	1944	.	+	0	ID=BBa_K175039;Name=BBa_K175039;Parent=BBa_K175042
BBa_K175050	.	engineered_region	1062	1944	.	+	0	ID=BBa_K145201;Name=BBa_K145201;Parent=BBa_K175039
BBa_K175050	.	CDS	1123	1807	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K145201
BBa_K175050	.	engineered_tag	1783	1807	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K175050	.	tag	1743	1776	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K175050	.	CDS	1126	1742	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K175050	.	ribosome_entry_site	1105	1116	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K145201
BBa_K175050	.	conserved_region	1109	1112	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K175050	.	promoter	1062	1096	.	+	0	ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_K145201
BBa_K175050	.	terminator	1816	1944	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K145201
BBa_K175050	.	terminator	1816	1895	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K175050	.	stem_loop	1827	1870	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K175050	.	engineered_region	1816	1895	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K175050	.	terminator	1904	1944	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K175050	.	engineered_region	1904	1944	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K175050	.	stop_codon	1937	1937	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K175050	.	stem_loop	1911	1930	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K175050	.	polyA_site	1931	1944	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K175050	.	sequence_feature	1024	1053	.	+	0	ID=BBa_K175027;Name=BBa_K175027;Parent=BBa_K175039
BBa_K175050	.	sequence_feature	1024	1053	.	+	0	ID=annotation2014585;Name=I-SceI homing endonuclease restriction site;Parent=BBa_K175027
BBa_K175050	.	engineered_region	1	92	.	+	0	ID=BBa_K175036;Name=BBa_K175036
BBa_K175050	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K175036
BBa_K175050	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K175050	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K175050	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K175050	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K175050	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K175050	.	sequence_feature	63	92	.	+	0	ID=BBa_K175027;Name=BBa_K175027;Parent=BBa_K175036
BBa_K175050	.	sequence_feature	63	92	.	+	0	ID=annotation2014585;Name=I-SceI homing endonuclease restriction site;Parent=BBa_K175027
>BBa_K175050
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagagttacgctagggataa
cagggtaatatagtactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtccca
attcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacg
gaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggtta
tggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttat
gtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgataccc
ttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaacta
taactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacatt
gaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagaca
accattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgt
aacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagta
gcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagtt
acgctagggataacagggtaatatagtactagagttgacagctagctcagtcctagggactatgctagctactagagaa
agaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcgga
atcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaata
agcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaag
ctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacat
ttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcac
tagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagt
cgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcac
caaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtg
ggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttata