BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation1701 1 RBS-1\Strong range1701 1 1 15 BBa_K1751003 1 BBa_K1751003 Promotor-RBS+RgPAL1 2015-08-15T11:00:00Z 2015-08-19T05:08:40Z iGEM's database and Rehmannia glutinosa Genome Promotor-RBS:This part contains a constitutive promoter and strong RBS. The strength of this RBS is considered relative to BBa_B0031, BBa_B0032, BBa_B0033 and BBa_B0034. While the promotor is a member of a family of constitutive promotor parts labelled J23100 through J23119. RgPAL1:The gene RgPAL1 can express Phenylalanine ammonia lyase&#65288;PAL&#65289;, which is an enzyme that catalyzes a reaction converting L-phenylalanine to ammonia and trans-cinnamic acid.PAL is the first and committed step in the phenyl propanoid pathway and is therefore involved in the biosynthesis of the polyphenol compounds. In our project, PAL plays an important role in L-phenylalanine???s deaminization to produce trans-cinnamic acid. Terminator &#65306;Using primers VR/VF2 to PCR B0010&B0012 will result in excess bands. false false _2173_ 28395 28395 9 Not in stock false Promotor-RBS+RgPAL1+terminator false Tianlai Xia component2436467 1 BBa_K1751000 component2436465 1 BBa_K847100 annotation2436467 1 BBa_K1751000 range2436467 1 65 2191 annotation2436465 1 BBa_K847100 range2436465 1 1 58 BBa_K1751000 1 BBa_K1751000 RgPAL1&#65306;phenylalanine ammonia lyase (PAL) coding region 2015-08-02T11:00:00Z 2015-08-19T04:47:14Z Rehmannia glutinosa. The gene RgPAL1 can express Phenylalanine ammonia lyase&#65288;PAL&#65289;, which is an??enzyme??that??catalyzes??a reaction converting L-phenylalanine??to ammonia??and??trans-cinnamic acid.PAL is the first and committed step in the??phenyl propanoid pathway??and is therefore involved in the??biosynthesis??of the??polyphenol??compounds. In our project, PAL plays an important role in L-phenylalanine???s deaminization to produce trans-cinnamic acid. false false _2173_ 28395 28395 9 false The gene RgPAL1 can express Phenylalanine ammonia lyase&#65288;PAL&#65289;. false Tianlai Xia annotation2434421 1 PAL coding region range2434421 1 1 2127 BBa_K847100 1 BBa_K847100 Promotor-RBS 2012-09-28T11:00:00Z 2015-05-08T01:13:34Z http://partsregistry.org/Part:BBa_B0030 http://partsregistry.org/Part:BBa_J23100 Released HQ 2013 This part contains a constitutive promoter and strong RBS. The strength of this RBS is considered relative to BBa_B0031, BBa_B0032, BBa_B0033 and BBa_B0034. While the promotor is a member of a family of constitutive promotor parts labelled J23100 through J23119. false false _1107_ 0 12286 9 In stock false None false Bella A Okiddy, Julia Borden, Michelle Yu component2210119 1 BBa_J23100 component2210121 1 BBa_B0030 annotation2210119 1 BBa_J23100 range2210119 1 1 35 annotation2210121 1 BBa_B0030 range2210121 1 44 58 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_K1751003_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatggagaatgggcaccaccactcgaacgggttgtgcgtggagactacgcgtgatccgttgaactgggtggcggcggcggagtcgctgaaggggagccacctggacgaggtgaagaggatggtggaggagttcaggaagccggcggtgaagctcggcggtgagagcctgactatagcgcaggtggcggcgatcgcggcgagggataatgcggtggcggtggagctggcggagacggcgcgtgcgggggttaaggcgagtagcgattgggttatggagagtatgaataaagggactgacagttatggagttacaacgggttttggtgccacgtcacataggaggactaaacaaggtggtgctcttcagaaggagctcattaggttcttgaatgccggaatattcggcaacggcacggaatctaaccacgcgctgccacactccgccacgagagccgccatgctcgtccgaatcaacacgctcctccaaggatattccggcatccgatttgaaatcctagaagccctaacaaaattcctcaaccacaacatcaccccctgtttgcccctccgcggcacgatcaccgcctccggcgacctcgtcccgctatcctacattgccgggcttttaacgggccggcccaactccaaggccgtcggcccaaacggcgaagccctcaacgccggcgaggctttcagcctcgccggcgttagcggcttcttcgagctgcaacccaaagaaggcctcgcgctagtcaacgggacagctgtcgggtccggattggcctcgatcgccctgtacgacgcgaacatcctcgccgtcctgtcggaagtgacgtcagtgattttcgctgaggtcatgaatgggaaacctgaatttacggatcatttgacacataagctgaaacatcaccctggccaaattgaggccgctgctataatggaacacattttagatggtagcgcgtacgttaaggctgctcagaaattgcacgaaaccgatccgttgcaaaaaccgaaacaggatcggtacgcgcttagaacgtcgcctcaatggctcggcccccaaatcgaagttatccgaaccgcgacgaaaatgatcgagcgggaaattaattcggttaacgacacacctctaatcgatgtctcgagaaataaagcgttacatggcggtaacttccagggcacgccaatcggggtatcgatggacaacaccagattggcgatagcagctatcggaaaattgatgttcgctcaattttccgagctggttaatgatttctacaacaatggattgccgtctaatctctctggcggtaggaatccgagcttggattacggtttcaaagggtccgaaatcgcgatggcttcgtattgttcggagcttcaatttttagctaatcctgttaccaatcatgtccaaagtgcagagcaacataaccaagatgtgaactcacttggattgatttcttccagaaagaccgtcgaggctctggatattctaaagctgatgtcatccacatatttaatcgcgctatgccaggccgtcgatttgaggcacttggaggagaatttgaggctttcagttaaaaacaccgttagccaagtggcgaagaggactctgacaatgggtattaatggcgaacttcatccgtcaagattctgcgagaaggatcttctccgtgtggtggaccgcgagtacgtgtttgcatacatcgacgatccgtgcagcgggacctaccccttgatgcagaagttgaggcaagttctcgtggaccacgcgttgaacaacggtgagagtgagaaaaacgtgagcacgtctatttttcaaaagatcgaggcgtttgaggtagagttgaaggcgatcttgcctaaagaggtcgagagtgcacggatcgcgttggagagtggaaatccggcgattggtaacaggattacggaatgcagatcgtatccgttgtacaagtttatcagagaggaacttgggacgaactacttgacgggcgaaaaggtcgtttctccgggggaggaatgtgataaggtgttcacagctttgagcaagggtttgattgttgatcctttgttgaagtgtcttgagggttggaatggtgcacctccccctatctgctag BBa_K1751000_sequence 1 atggagaatgggcaccaccactcgaacgggttgtgcgtggagactacgcgtgatccgttgaactgggtggcggcggcggagtcgctgaaggggagccacctggacgaggtgaagaggatggtggaggagttcaggaagccggcggtgaagctcggcggtgagagcctgactatagcgcaggtggcggcgatcgcggcgagggataatgcggtggcggtggagctggcggagacggcgcgtgcgggggttaaggcgagtagcgattgggttatggagagtatgaataaagggactgacagttatggagttacaacgggttttggtgccacgtcacataggaggactaaacaaggtggtgctcttcagaaggagctcattaggttcttgaatgccggaatattcggcaacggcacggaatctaaccacgcgctgccacactccgccacgagagccgccatgctcgtccgaatcaacacgctcctccaaggatattccggcatccgatttgaaatcctagaagccctaacaaaattcctcaaccacaacatcaccccctgtttgcccctccgcggcacgatcaccgcctccggcgacctcgtcccgctatcctacattgccgggcttttaacgggccggcccaactccaaggccgtcggcccaaacggcgaagccctcaacgccggcgaggctttcagcctcgccggcgttagcggcttcttcgagctgcaacccaaagaaggcctcgcgctagtcaacgggacagctgtcgggtccggattggcctcgatcgccctgtacgacgcgaacatcctcgccgtcctgtcggaagtgacgtcagtgattttcgctgaggtcatgaatgggaaacctgaatttacggatcatttgacacataagctgaaacatcaccctggccaaattgaggccgctgctataatggaacacattttagatggtagcgcgtacgttaaggctgctcagaaattgcacgaaaccgatccgttgcaaaaaccgaaacaggatcggtacgcgcttagaacgtcgcctcaatggctcggcccccaaatcgaagttatccgaaccgcgacgaaaatgatcgagcgggaaattaattcggttaacgacacacctctaatcgatgtctcgagaaataaagcgttacatggcggtaacttccagggcacgccaatcggggtatcgatggacaacaccagattggcgatagcagctatcggaaaattgatgttcgctcaattttccgagctggttaatgatttctacaacaatggattgccgtctaatctctctggcggtaggaatccgagcttggattacggtttcaaagggtccgaaatcgcgatggcttcgtattgttcggagcttcaatttttagctaatcctgttaccaatcatgtccaaagtgcagagcaacataaccaagatgtgaactcacttggattgatttcttccagaaagaccgtcgaggctctggatattctaaagctgatgtcatccacatatttaatcgcgctatgccaggccgtcgatttgaggcacttggaggagaatttgaggctttcagttaaaaacaccgttagccaagtggcgaagaggactctgacaatgggtattaatggcgaacttcatccgtcaagattctgcgagaaggatcttctccgtgtggtggaccgcgagtacgtgtttgcatacatcgacgatccgtgcagcgggacctaccccttgatgcagaagttgaggcaagttctcgtggaccacgcgttgaacaacggtgagagtgagaaaaacgtgagcacgtctatttttcaaaagatcgaggcgtttgaggtagagttgaaggcgatcttgcctaaagaggtcgagagtgcacggatcgcgttggagagtggaaatccggcgattggtaacaggattacggaatgcagatcgtatccgttgtacaagtttatcagagaggaacttgggacgaactacttgacgggcgaaaaggtcgtttctccgggggaggaatgtgataaggtgttcacagctttgagcaagggtttgattgttgatcctttgttgaagtgtcttgagggttggaatggtgcacctccccctatctgctag BBa_B0030_sequence 1 attaaagaggagaaa BBa_K847100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z