LOCUS       BBa_K1755005             853 bp    DNA     linear       12-Jun-2024
DEFINITION  strong promoter + medium RBS +ribB + terminator
ACCESSION   BBa_K1755005
VERSION     BBa_K1755005.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23104
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     misc_feature    1..56
                     /label=BBa_K608003
     stem_loop       736..779
                     /label=stem_loop
     misc_feature    725..804
                     /label=BBa_B0010
     terminator      725..804
                     /label=BBa_B0010
     stem_loop       820..839
                     /label=T7 TE
     misc_feature    846..846
                     /label=stop
     polyA_site      840..853
                     /label=polya
     misc_feature    813..853
                     /label=BBa_B0012
     terminator      813..853
                     /label=BBa_B0012
     CDS             63..716
                     /label=BBa_K1755001
     stem_loop       732..751
                     /label=T7 TE
     misc_feature    758..758
                     /label=stop
     polyA_site      752..765
                     /label=polya
     misc_feature    725..765
                     /label=BBa_B0012
     terminator      725..765
                     /label=BBa_B0012
     stem_loop       786..808
                     /label=stem_loop
     misc_feature    774..819
                     /label=BBa_B0011
     terminator      774..819
                     /label=BBa_B0011
     terminator      725..819
                     /label=BBa_B0014
     CDS             63..853
                     /label=BBa_K1755002
ORIGIN
        1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagtcacaca ggaaagtact
       61 agatgaatca gacgctactt tcctcttttg gtacgccttt cgaacgtgtt gaaaatgcac
      121 tggctgcgct gcgtgaagga cgcggtgtaa tggtgcttga tgatgaagac cgtgaaaacg
      181 aaggtgatat gatcttcccg gcagaaacca tgactgttga gcagatggcg ctgaccattc
      241 gccacggtag cggtattgtt tgcctgtgca ttactgaaga tcgccgtaaa caactcgatc
      301 tgccaatgat ggtagaaaat aacaccagcg cctatggcac cggttttacc gtgaccattg
      361 aagcagctga aggtgtgact accggtgttt ctgccgctga ccgtattacg accgttcgcg
      421 cagcgattgc cgatggcgca aaaccgtcag atctgaatcg tcctggccac gttttcccac
      481 ttcgcgctca ggcaggtggt gtactgacgc gtggcggtca tactgaagca actattgatc
      541 tgatgacgct ggcaggcttt aaaccggctg gtgtactgtg tgagctgact aatgacgatg
      601 gcacgatggc gcgtgcacca gagtgtattg agtttgccaa taaacacaat atggcgctcg
      661 tgactattga agacctggtg gcataccgtc aggcacatga gcgtaaagcc agctgatact
      721 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      781 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
      841 ttctgcgttt ata
//