BBa_B0031
1
BBa_B0031
RBS.2 (weak) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
<P> <P>Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Cho</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23316
1
conserved
range23316
1
7
10
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation7019
1
BBa_B0011
range7019
1
1
46
annotation1683
1
stem_loop
range1683
1
13
35
BBa_K608007
1
BBa_K608007
medium Promoter , weak RBS
2011-09-14T11:00:00Z
2015-05-08T01:12:52Z
composite
Released HQ 2013
medium Promotor , weak RBS
false
false
_780_
0
9115
9
In stock
false
composite
false
Julia M??ller
component2128663
1
BBa_J23110
component2128665
1
BBa_B0031
annotation2128663
1
BBa_J23110
range2128663
1
1
35
annotation2128665
1
BBa_B0031
range2128665
1
44
57
BBa_K1755001
1
BBa_K1755001
ribB coding sequence
2015-04-19T11:00:00Z
2015-05-08T01:11:00Z
You can find it from almost all E.coli cells. More infomation you can find from this website:
http://www.ncbi.nlm.nih.gov/gene/12931678
ribB，the 3,4 dihydroxy-2-butanone-4-phosphate synthase。
We get this part from E.coli genome by PCR.
It can increase the electric output by synthesis of riboflavin.
false
false
_2177_
0
21246
9
Not in stock
false
The primer is below:
F:5'-3' CGT TCTAGA TGAATCAGACGCT 60.46 45 22
R:5'-3' GGT ACTAGT TCAGCTGGCTTAC 60.66 50 22
false
Haotian Wang
BBa_J23110
1
BBa_J23110
constitutive promoter family member
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
Later
Later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K1755002
1
BBa_K1755002
ribB+B0015
2015-04-19T11:00:00Z
2015-06-01T08:38:11Z
ribB with double terminators
false
false
_2177_
21246
21246
9
Not in stock
false
false
Haotian Wang
component2432406
1
BBa_K1755001
component2432413
1
BBa_B0014
annotation2432406
1
BBa_K1755001
range2432406
1
1
654
annotation2432413
1
BBa_B0014
range2432413
1
663
757
BBa_B0014
1
BBa_B0014
double terminator (B0012-B0011)
2003-07-15T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0012 and BBa_B0011
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component939311
1
BBa_B0011
component939303
1
BBa_B0012
annotation939311
1
BBa_B0011
range939311
1
50
95
annotation939303
1
BBa_B0012
range939303
1
1
41
BBa_K1755009
1
BBa_K1755009
Medium Promoter +weak RBS+ribB+ terminator
2015-07-02T11:00:00Z
2015-08-28T01:29:26Z
false
false
_2177_
21246
21246
9
false
false
Haotian Wang
component2432959
1
BBa_K1755002
component2432950
1
BBa_K608007
annotation2432959
1
BBa_K1755002
range2432959
1
64
820
annotation2432950
1
BBa_K608007
range2432950
1
1
57
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
BBa_K608007_sequence
1
tttacggctagctcagtcctaggtacaatgctagctactagagtcacacaggaaacc
BBa_B0014_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_K1755001_sequence
1
atgaatcagacgctactttcctcttttggtacgcctttcgaacgtgttgaaaatgcactggctgcgctgcgtgaaggacgcggtgtaatggtgcttgatgatgaagaccgtgaaaacgaaggtgatatgatcttcccggcagaaaccatgactgttgagcagatggcgctgaccattcgccacggtagcggtattgtttgcctgtgcattactgaagatcgccgtaaacaactcgatctgccaatgatggtagaaaataacaccagcgcctatggcaccggttttaccgtgaccattgaagcagctgaaggtgtgactaccggtgtttctgccgctgaccgtattacgaccgttcgcgcagcgattgccgatggcgcaaaaccgtcagatctgaatcgtcctggccacgttttcccacttcgcgctcaggcaggtggtgtactgacgcgtggcggtcatactgaagcaactattgatctgatgacgctggcaggctttaaaccggctggtgtactgtgtgagctgactaatgacgatggcacgatggcgcgtgcaccagagtgtattgagtttgccaataaacacaatatggcgctcgtgactattgaagacctggtggcataccgtcaggcacatgagcgtaaagccagctga
BBa_K1755002_sequence
1
atgaatcagacgctactttcctcttttggtacgcctttcgaacgtgttgaaaatgcactggctgcgctgcgtgaaggacgcggtgtaatggtgcttgatgatgaagaccgtgaaaacgaaggtgatatgatcttcccggcagaaaccatgactgttgagcagatggcgctgaccattcgccacggtagcggtattgtttgcctgtgcattactgaagatcgccgtaaacaactcgatctgccaatgatggtagaaaataacaccagcgcctatggcaccggttttaccgtgaccattgaagcagctgaaggtgtgactaccggtgtttctgccgctgaccgtattacgaccgttcgcgcagcgattgccgatggcgcaaaaccgtcagatctgaatcgtcctggccacgttttcccacttcgcgctcaggcaggtggtgtactgacgcgtggcggtcatactgaagcaactattgatctgatgacgctggcaggctttaaaccggctggtgtactgtgtgagctgactaatgacgatggcacgatggcgcgtgcaccagagtgtattgagtttgccaataaacacaatatggcgctcgtgactattgaagacctggtggcataccgtcaggcacatgagcgtaaagccagctgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_K1755009_sequence
1
tttacggctagctcagtcctaggtacaatgctagctactagagtcacacaggaaacctactagatgaatcagacgctactttcctcttttggtacgcctttcgaacgtgttgaaaatgcactggctgcgctgcgtgaaggacgcggtgtaatggtgcttgatgatgaagaccgtgaaaacgaaggtgatatgatcttcccggcagaaaccatgactgttgagcagatggcgctgaccattcgccacggtagcggtattgtttgcctgtgcattactgaagatcgccgtaaacaactcgatctgccaatgatggtagaaaataacaccagcgcctatggcaccggttttaccgtgaccattgaagcagctgaaggtgtgactaccggtgtttctgccgctgaccgtattacgaccgttcgcgcagcgattgccgatggcgcaaaaccgtcagatctgaatcgtcctggccacgttttcccacttcgcgctcaggcaggtggtgtactgacgcgtggcggtcatactgaagcaactattgatctgatgacgctggcaggctttaaaccggctggtgtactgtgtgagctgactaatgacgatggcacgatggcgcgtgcaccagagtgtattgagtttgccaataaacacaatatggcgctcgtgactattgaagacctggtggcataccgtcaggcacatgagcgtaaagccagctgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0031_sequence
1
tcacacaggaaacc
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_J23110_sequence
1
tttacggctagctcagtcctaggtacaatgctagc
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z