LOCUS       BBa_K1755302             888 bp    DNA     linear       27-Jun-2024
DEFINITION  copper promoter + RBS + ribB + terminator
ACCESSION   BBa_K1755302
VERSION     BBa_K1755302.1  
FEATURES             Location/Qualifiers
     promoter        1..43
                     /label=-35 and -10 region
     RBS             44..71
                     /label=RBS site
     promoter        1..71
                     /label=BBa_K190024
     misc_feature    84..87
                     /label=conserved
     RBS             80..91
                     /label=BBa_B0034
     CDS             98..751
                     /label=BBa_K1755001
     stem_loop       771..814
                     /label=stem_loop
     misc_feature    760..839
                     /label=BBa_B0010
     terminator      760..839
                     /label=BBa_B0010
     stem_loop       855..874
                     /label=T7 TE
     misc_feature    881..881
                     /label=stop
     polyA_site      875..888
                     /label=polya
     misc_feature    848..888
                     /label=BBa_B0012
     terminator      848..888
                     /label=BBa_B0012
     RBS             80..92
                     /label=RBS-3\Weak
     RBS             86..89
                     /label=RBS
     misc_feature    80..92
                     /label=BBa_B0032
     RBS             80..92
                     /label=BBa_B0032
     CDS             99..752
                     /label=BBa_K1755001
     stem_loop       768..787
                     /label=T7 TE
     misc_feature    794..794
                     /label=stop
     polyA_site      788..801
                     /label=polya
     misc_feature    761..801
                     /label=BBa_B0012
     terminator      761..801
                     /label=BBa_B0012
     stem_loop       822..844
                     /label=stem_loop
     misc_feature    810..855
                     /label=BBa_B0011
     terminator      810..855
                     /label=BBa_B0011
     terminator      761..855
                     /label=BBa_B0014
     CDS             99..855
                     /label=BBa_K1755002
     misc_feature    80..888
                     /label=BBa_K1755003
ORIGIN
        1 ttgaccttcc cgtaagggga aggactatgc tcaacgtttg attttgtttc gcctgcttaa
       61 gaataaggaa atactagaga aagaggagaa atactagatg aatcagacgc tactttcctc
      121 ttttggtacg cctttcgaac gtgttgaaaa tgcactggct gcgctgcgtg aaggacgcgg
      181 tgtaatggtg cttgatgatg aagaccgtga aaacgaaggt gatatgatct tcccggcaga
      241 aaccatgact gttgagcaga tggcgctgac cattcgccac ggtagcggta ttgtttgcct
      301 gtgcattact gaagatcgcc gtaaacaact cgatctgcca atgatggtag aaaataacac
      361 cagcgcctat ggcaccggtt ttaccgtgac cattgaagca gctgaaggtg tgactaccgg
      421 tgtttctgcc gctgaccgta ttacgaccgt tcgcgcagcg attgccgatg gcgcaaaacc
      481 gtcagatctg aatcgtcctg gccacgtttt cccacttcgc gctcaggcag gtggtgtact
      541 gacgcgtggc ggtcatactg aagcaactat tgatctgatg acgctggcag gctttaaacc
      601 ggctggtgta ctgtgtgagc tgactaatga cgatggcacg atggcgcgtg caccagagtg
      661 tattgagttt gccaataaac acaatatggc gctcgtgact attgaagacc tggtggcata
      721 ccgtcaggca catgagcgta aagccagctg atactagagc caggcatcaa ataaaacgaa
      781 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
      841 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//