BBa_K1758101 1 BBa_K1758101 Translation enhancing 5-UTR + sfGFP 2015-08-16T11:00:00Z 2015-09-19T02:13:09Z This part was created by shortening <a href="http://parts.igem.org/Part:BBa_I746909">BBa_I746909</a> and simultaneously adding 5'-UTR via Gibson primers. '''Amplification with Gibson-primers:''' This part includes a translation enhancing sequence, 5'-UTR, in front of sfGFP coding sequence (BBa_I746916). It also contains a double terminator made of <a href"http://parts.igem.org/wiki/index.php?title=Part:BBa_B0010">B0010</a> and <a href"http://parts.igem.org/wiki/index.php?title=Part:BBa_B0012">B0012</a>. The translation enhancing sequence improves the binding of the mRNA to the ribosome (<a href="http://www.ncbi.nlm.nih.gov/pubmed/2676996">Olins et al. 1989</a>) and is especially helpful in <i>in vitro</i> cell free protein synthesis. The sequence contains a spacer of 10 adenine bases which has been shown to further enhance translation efficiency (<a href="http://www.ncbi.nlm.nih.gov/pubmed/23927491">Takahashi et al. 2013</a>). false false _2180_ 26563 26563 9 false <a href="http://www.ncbi.nlm.nih.gov/pubmed/23654270">Lentini et al. 2013</a> showed that the scar which is created by standard biobrick assembly is disadvantageous for <i>in vitro</i> translation when occuring between RBS and start codon. Therefore we changed the sequence between RBS and start codon for other parts. false Team Bielefeld-CeBiTec 2015 annotation2435912 1 B0010 range2435912 1 775 854 annotation2435913 1 B0012 range2435913 1 863 903 annotation2439117 1 I746916 range2439117 1 47 763 annotation2435911 1 5'-UTR with g10 RBS range2435911 1 1 46 annotation2436053 1 stop range2436053 1 761 766 annotation2435910 1 sfGFP range2435910 1 47 763 BBa_I719005 1 pT7 T7 Promoter 2007-10-23T11:00:00Z 2015-08-31T04:07:53Z --- Released HQ 2013 Just a T7 Promoter false false _128_ 0 2097 9 In stock true None true Imperial 2007 BBa_K1758102 1 BBa_K1758102 Translation enhancing 5-UTR + sfGFP under control of T7 promoter 2015-08-17T11:00:00Z 2015-09-19T01:12:55Z This part was created by adding 5'-UTR to [http://parts.igem.org/Part:BBa_I746909 BBa_I746909] via Gibson primers. sfGFP ([http://parts.igem.org/wiki/index.php?title=Part:BBa_I746916 BBa_I746916]) under control of the T7 promoter ([http://parts.igem.org/Part:BBa_I719005 BBa_I719005]). The part includes a translation enhancing sequence, 5'-UTR, which improves the binding of the mRNA to the ribosome ([http://www.ncbi.nlm.nih.gov/pubmed/2676996 Olins et al. 1989]) and is especially helpful in ''in vitro'' cell free protein synthesis. The 5'-UTR sequence contains a spacer of 10 adenine bases which has been shown to further enhance translation efficiency ([http://www.ncbi.nlm.nih.gov/pubmed/23927491 Takahashi et al. 2013]). The part also contains a double terminator made of [http://parts.igem.org/wiki/index.php?title=Part:BBa_B0010 B0010] and [http://parts.igem.org/wiki/index.php?title=Part:BBa_B0012 B0012]. false false _2180_ 26563 26563 9 true [http://www.ncbi.nlm.nih.gov/pubmed/23654270 Lentini et al. 2013] showed that the scar which is created by standard biobrick assembly is disadvantageous for ''in vitro'' translation when occuring between RBS and start codon. false Team Bielefeld-CeBiTec 2015 component2439125 1 BBa_K1758101 component2439118 1 BBa_I719005 annotation2439125 1 BBa_K1758101 range2439125 1 24 927 annotation2439118 1 BBa_I719005 range2439118 1 1 23 BBa_I719005_sequence 1 taatacgactcactatagggaga BBa_K1758101_sequence 1 aataattttgtttaactttaaaaaaaaaaaagaaggagatatacatatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat BBa_K1758102_sequence 1 taatacgactcactatagggagaaataattttgtttaactttaaaaaaaaaaaagaaggagatatacatatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z