LOCUS       BBa_K1758105             895 bp    DNA     linear       22-Dec-2024
DEFINITION  mRFP generator under control of T7 promoter
ACCESSION   BBa_K1758105
VERSION     BBa_K1758105.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=BBa_I719005
     RBS             32..46
                     /label=RBS-1\Strong
     RBS             39..43
                     /label=RBS
     misc_feature    32..46
                     /label=BBa_B0030
     RBS             32..46
                     /label=BBa_B0030
     CDS             53..727
                     /label=mrfp1
     misc_feature    734..758
                     /label=Help:Barcodes
     CDS             53..758
                     /label=BBa_E1010
     stem_loop       778..821
                     /label=stem_loop
     misc_feature    767..846
                     /label=BBa_B0010
     terminator      767..846
                     /label=BBa_B0010
     stem_loop       862..881
                     /label=T7 TE
     misc_feature    888..888
                     /label=stop
     polyA_site      882..895
                     /label=polya
     misc_feature    855..895
                     /label=BBa_B0012
     terminator      855..895
                     /label=BBa_B0012
     terminator      767..895
                     /label=BBa_B0015
     misc_feature    32..895
                     /label=BBa_K516030
ORIGIN
        1 taatacgact cactataggg agatactaga gattaaagag gagaaatact agatggcttc
       61 ctccgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa
      121 cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac
      181 cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc
      241 gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct
      301 gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg
      361 tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa
      421 actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg
      481 ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat
      541 gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc
      601 taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc
      661 ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac
      721 cggtgcttaa taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata
      781 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
      841 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//