BBa_K864100
1
SYFP2
Super Yellow Fluorescent Protein 2 (SYFP2)
2012-09-23T11:00:00Z
2016-01-21T02:29:24Z
Amino acid sequence taken from the article by Kremers et al 2006.
Released HQ 2013
This part codes for the bright yellow fluorescent protein SYFP2. SYFP2 is a GFP based monomeric protein with a narrow fluorescence emission spectrum with a maximum at 515 nm. Bacteria expressing SYFP2 are reported to be 12 times brighter than those expressing EYFP(Q69K) and almost 2-fold brighter than bacteria expressing Venus (Ref. 10.1021/bi0516273). Codon optimized for expression in E coli by DNA 2.0. Mutations compared to wtGFP amino acid sequence (GenBank Accession number M62653) are F46L F64L S65G S72A M153T V163A S175G T203Y A206K.
false
false
_1124_
4206
10137
9
In stock
false
The sequence has been codon optimized for expression in E coli by DNA 2.0.
false
Erik Gullberg
annotation2196221
1
SYFP2
range2196221
1
1
717
BBa_K525998
1
pT7
Promoter T7 and RBS
2011-09-12T11:00:00Z
2015-05-08T01:12:35Z
Synthesis
Released HQ 2013
Promoter T7 and RBS
false
false
_690_
0
8543
9
In stock
false
Synthesis
false
Anna Drong
annotation2129432
1
T7 promoter
range2129432
1
1
20
annotation2129433
1
RBS
range2129433
1
24
27
annotation2127788
1
B0034
range2127788
1
21
32
BBa_K1758107
1
BBa_K1758107
SYFP2 under T7 promoter + RBS
2015-08-28T11:00:00Z
2015-09-18T04:42:23Z
3A assembly of [http://parts.igem.org/Part:BBa_K525998 BBa_K525998] and [http://parts.igem.org/Part:BBa_K864100 BBa_K864100]
SYFP2 is synthesized when T7-polymerase is induced.
false
false
_2180_
27935
26563
9
false
---
false
Team Bielefeld-CeBiTec 2015
component2439949
1
BBa_K864100
component2439947
1
BBa_K525998
annotation2439947
1
BBa_K525998
range2439947
1
1
32
annotation2439949
1
BBa_K864100
range2439949
1
39
761
BBa_K864100_sequence
1
atggttagcaagggcgaagaactttttacaggcgtagtaccgatcttagttgaattagacggcgacgttaacggtcataagtttagcgtgagcggtgagggtgaaggtgacgcaacttacggcaagctgaccctgaagctgatttgcacgacgggtaagctgccggtcccgtggcctaccctggtcacgaccttgggttatggcgttcagtgtttcgcgcgttatccggaccacatgaaacaacacgatttctttaagagcgcgatgccagaaggctatgtgcaggagcgtacgatctttttcaaagacgacggtaactacaagacgcgtgccgaagtcaaattcgaaggcgacaccctggtgaatcgcattgagctgaagggtattgatttcaaagaggatggcaatatcctgggtcacaagctggagtacaattacaattcccacaacgtttacatcaccgcagataaacagaaaaatggcatcaaagcgaatttcaaaatccgtcacaacattgaggacggtggtgttcaactggcggatcattaccagcaaaacaccccgattggtgacggtccggtcctgttgccggataaccattatctgtcttaccaaagcaaactgagcaaagatccgaacgagaagcgcgaccacatggtgctgctggagtttgtgaccgctgccggtattaccctgggtatggatgagctgtataaataataa
BBa_K525998_sequence
1
taatacgactcactatagggaaagaggagaaa
BBa_K1758107_sequence
1
taatacgactcactatagggaaagaggagaaatactagatggttagcaagggcgaagaactttttacaggcgtagtaccgatcttagttgaattagacggcgacgttaacggtcataagtttagcgtgagcggtgagggtgaaggtgacgcaacttacggcaagctgaccctgaagctgatttgcacgacgggtaagctgccggtcccgtggcctaccctggtcacgaccttgggttatggcgttcagtgtttcgcgcgttatccggaccacatgaaacaacacgatttctttaagagcgcgatgccagaaggctatgtgcaggagcgtacgatctttttcaaagacgacggtaactacaagacgcgtgccgaagtcaaattcgaaggcgacaccctggtgaatcgcattgagctgaagggtattgatttcaaagaggatggcaatatcctgggtcacaagctggagtacaattacaattcccacaacgtttacatcaccgcagataaacagaaaaatggcatcaaagcgaatttcaaaatccgtcacaacattgaggacggtggtgttcaactggcggatcattaccagcaaaacaccccgattggtgacggtccggtcctgttgccggataaccattatctgtcttaccaaagcaaactgagcaaagatccgaacgagaagcgcgaccacatggtgctgctggagtttgtgaccgctgccggtattaccctgggtatggatgagctgtataaataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z