BBa_K1758120 1 BBa_K1758120 RNase E regulating protein RraA 2015-08-12T11:00:00Z 2015-09-18T07:05:34Z RraA was obtained via PCR on ''E. coli'' K12 genomic DNA. The product of the RraA-gene has been reported to interact with RNase E from ''E. coli'' and to alternate its activity. Airen showed that if RraA-protein is added to a cell free protein synthesis reaction, the productivity raises about 30% (Airen, 2011). He postulated that activity of RNase E in the reaction is lowered due to the interaction with RraA-protein, therefore mRNA-levels are stabilized. RNase E is brought to the ''in vitro'' reaction by ''E. coli'' cell extract itself. false false _2180_ 26563 26563 9 true '''Amplification with Gibson-primer:''' *Amplification of regulator of ribonuclease activity A (''RraA''):<br> '''Forward primer RraA'''<br> 5'-ATGAAATACGATACTTCCGAGCTTTG-3'<br> '''Reverse primer RraA'''<br> 5'-TCATTCAATATCCAGCGGATCTTC-3'<br> *Amplification of the pSB1C3-Backbone:<br> '''Forward primer pSB1C3 RraA(43 bp)'''<br> 5'-TCAGAAGATCCGCTGGATATTGAATGATACTAGTAGCGGCCGCTGCAG-3'<br> '''Reverse primer pSB1C3 RraA(43 bp)'''<br> 5'-TTCTTCCAGTTGTTCCGGGTTTGCCATCTCTAGAAGCGGCCGCGAATTCC-3'<br> '''Ligation via [http://2015.igem.org/Team:Bielefeld-CeBiTec/Protocols Gibson-Assembly].''' false Team Bielefeld-CeBiTec 2015 annotation2435621 1 rraA (menG) range2435621 1 1 486 BBa_K1758120_sequence 1 atgaaatacgatacttccgagctttgtgacatctatcaagaagatgttaacgtcgtggaaccgctgttctccaactttggcggacgggcgtcgtttggcggacaaataatcacggtaaaatgtttcgaggacaacgggttgctgtacgatctgctcgaacagaatggccgtggtcgtgttcttgtcgtcgatggcggtggttctgttcgtcgcgcactggtcgatgctgaactggcgcgtctggcagtacaaaatgaatgggaaggtctggtcatttacggcgcggtgcgtcaggtagatgacctggaagagttggatatcggcatccaggcgatggcggcaattccggttggtgccgctggcgaaggcattggcgaaagcgatgtccgcgtcaattttggcggtgtcaccttcttctccggcgaccatctttatgccgacaataccgggattattctttcagaagatccgctggatattgaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z