LOCUS BBa_K1758122 756 bp DNA linear 22-Nov-2024 DEFINITION RraA with N-terminal His-TEV-tag under T7 promoter ACCESSION BBa_K1758122 VERSION BBa_K1758122.1 FEATURES Location/Qualifiers misc_feature 1..23 /label=I719005 promoter 1..23 /label=T7 RBS 32..43 /label=RBS misc_feature 32..43 /label=B0034 misc_feature 62..79 /label=6xHis misc_feature 101..118 /label=TEV cleavage site CDS 125..619 /label=RraA (menG) misc_feature 628..707 /label=B0010 misc_feature 716..756 /label=B0012 ORIGIN 1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tgtcgtacta 61 ccatcaccat caccatcacg attacgatat cccaacgacc gaaaacctgt attttcaggg 121 cgccatgaaa tacgatactt ccgagctttg tgacatctat caagaagatg ttaacgtcgt 181 ggaaccgctg ttctccaact ttggcggacg ggcgtcgttt ggcggacaaa taatcacggt 241 aaaatgtttc gaggacaacg ggttgctgta cgatctgctc gaacagaatg gccgtggtcg 301 tgttcttgtc gtcgatggcg gtggttctgt tcgtcgcgca ctggtcgatg ctgaactggc 361 gcgtctggca gtacaaaatg aatgggaagg tctggtcatt tacggcgcgg tgcgtcaggt 421 agatgacctg gaagagttgg atatcggcat ccaggcgatg gcggcaattc cggttggtgc 481 cgctggcgaa ggcattggcg aaagcgatgt ccgcgtcaat tttggcggtg tcaccttctt 541 ctccggcgac catctttatg ccgacaatac cgggattatt ctttcagaag attcagaaga 601 tccgctggat attgaatgat actagagcca ggcatcaaat aaaacgaaag gctcagtcga 661 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 721 ctggctcacc ttcgggtggg cctttctgcg tttata //