LOCUS       BBa_K1758122             756 bp    DNA     linear       23-Dec-2024
DEFINITION  RraA with N-terminal His-TEV-tag under T7 promoter
ACCESSION   BBa_K1758122
VERSION     BBa_K1758122.1  
FEATURES             Location/Qualifiers
     misc_feature    1..23
                     /label=I719005
     promoter        1..23
                     /label=T7
     RBS             32..43
                     /label=RBS
     misc_feature    32..43
                     /label=B0034
     misc_feature    62..79
                     /label=6xHis
     misc_feature    101..118
                     /label=TEV cleavage site
     CDS             125..619
                     /label=RraA (menG)
     misc_feature    628..707
                     /label=B0010
     misc_feature    716..756
                     /label=B0012
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tgtcgtacta
       61 ccatcaccat caccatcacg attacgatat cccaacgacc gaaaacctgt attttcaggg
      121 cgccatgaaa tacgatactt ccgagctttg tgacatctat caagaagatg ttaacgtcgt
      181 ggaaccgctg ttctccaact ttggcggacg ggcgtcgttt ggcggacaaa taatcacggt
      241 aaaatgtttc gaggacaacg ggttgctgta cgatctgctc gaacagaatg gccgtggtcg
      301 tgttcttgtc gtcgatggcg gtggttctgt tcgtcgcgca ctggtcgatg ctgaactggc
      361 gcgtctggca gtacaaaatg aatgggaagg tctggtcatt tacggcgcgg tgcgtcaggt
      421 agatgacctg gaagagttgg atatcggcat ccaggcgatg gcggcaattc cggttggtgc
      481 cgctggcgaa ggcattggcg aaagcgatgt ccgcgtcaat tttggcggtg tcaccttctt
      541 ctccggcgac catctttatg ccgacaatac cgggattatt ctttcagaag attcagaaga
      601 tccgctggat attgaatgat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
      661 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      721 ctggctcacc ttcgggtggg cctttctgcg tttata
//