BBa_K1758122 1 BBa_K1758122 RraA with N-terminal His-TEV-tag under T7 promoter 2015-08-26T11:00:00Z 2015-09-18T06:41:46Z This part was obtained from ''E. coli'' K12 genomic DNA and cloned to pSB1C3 with Gibson assembly. With this part you can overproduce purify ''E. coli'' protein [http://parts.igem.org/Part:BBa_K1758120 RraA]. The part is made up of T7-promoter ([http://parts.igem.org/Part:BBa_I719005 BBa_I719005]) and [http://parts.igem.org/Part:BBa_K1758120 RraA] fused to an N-terminal His-tag and TEV cleavage site. false false _2180_ 26563 26563 9 false The purification sequence is based on the corresponding sequence in [http://tools.thermofisher.com/content/sfs/vectors/pproexhtc_mcs.pdf pProExHTb] false Team Bielefeld-CeBiTec 2015 annotation2439131 1 TEV cleavage site range2439131 1 101 118 annotation2439133 1 B0010 range2439133 1 628 707 annotation2439132 1 RraA (menG) range2439132 1 125 619 annotation2439128 1 RBS range2439128 1 32 43 annotation2439126 1 I719005 range2439126 1 1 23 annotation2439127 1 T7 range2439127 1 1 23 annotation2439134 1 B0012 range2439134 1 716 756 annotation2439130 1 6xHis range2439130 1 62 79 annotation2439129 1 B0034 range2439129 1 32 43 BBa_K1758122_sequence 1 taatacgactcactatagggagatactagagaaagaggagaaatactagatgtcgtactaccatcaccatcaccatcacgattacgatatcccaacgaccgaaaacctgtattttcagggcgccatgaaatacgatacttccgagctttgtgacatctatcaagaagatgttaacgtcgtggaaccgctgttctccaactttggcggacgggcgtcgtttggcggacaaataatcacggtaaaatgtttcgaggacaacgggttgctgtacgatctgctcgaacagaatggccgtggtcgtgttcttgtcgtcgatggcggtggttctgttcgtcgcgcactggtcgatgctgaactggcgcgtctggcagtacaaaatgaatgggaaggtctggtcatttacggcgcggtgcgtcaggtagatgacctggaagagttggatatcggcatccaggcgatggcggcaattccggttggtgccgctggcgaaggcattggcgaaagcgatgtccgcgtcaattttggcggtgtcaccttcttctccggcgaccatctttatgccgacaataccgggattattctttcagaagattcagaagatccgctggatattgaatgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z