LOCUS       BBa_K1758202            1877 bp    DNA     linear       08-May-2024
DEFINITION  His-tagged lac repressor fused to superfolder GFP under the 
            control of the T7 promoter
ACCESSION   BBa_K1758202
VERSION     BBa_K1758202.1  
FEATURES             Location/Qualifiers
     CDS             39..1127
                     /label=lacI
     misc_feature    1..32
                     /label=BBa_K525998
     RBS             24..27
                     /label=RBS
     promoter        1..23
                     /label=T7 promoter
     misc_feature    1854..1877
                     /label=His-Tag
     misc_feature    21..32
                     /label=BBa_B0034
     CDS             1134..1853
                     /label=sfGFP
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagat ggtgaatgtg aaaccagtaa
       61 cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga
      121 accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc
      181 tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg
      241 gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat
      301 ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg
      361 aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta
      421 actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg
      481 cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag
      541 acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt
      601 tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc
      661 tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg
      721 gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg
      781 ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg
      841 gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc
      901 cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc
      961 tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga
     1021 aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     1081 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagacc ggcatgcgta
     1141 aaggcgaaga gctgttcact ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca
     1201 acggtcataa gttttccgtg cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga
     1261 cgctgaagtt catctgtact actggtaaac tgccggtacc ttggccgact ctggtaacga
     1321 cgctgactta tggtgttcag tgctttgctc gttatccgga ccatatgaag cagcatgact
     1381 tcttcaagtc cgccatgccg gaaggctatg tgcaggaacg cacgatttcc tttaaggatg
     1441 acggcacgta caaaacgcgt gcggaagtga aatttgaagg cgataccctg gtaaaccgca
     1501 ttgagctgaa aggcattgac tttaaagaag acggcaatat cctgggccat aagctggaat
     1561 acaattttaa cagccacaat gtttacatca ccgccgataa acaaaaaaat ggcattaaag
     1621 cgaattttaa aattcgccac aacgtggagg atggcagcgt gcagctggct gatcactacc
     1681 agcaaaacac tccaatcggt gatggtcctg ttctgctgcc agacaatcac tatctgagca
     1741 cgcaaagcgt tctgtctaaa gatccgaacg agaaacgcga tcatatggtt ctgctggagt
     1801 tcgtaaccgc agcgggcatc acgcatggta tggatgaact gtacaaacac catcaccatc
     1861 accatcacca ttaataa
//