LOCUS BBa_K1758203 1283 bp DNA linear 22-Nov-2024 DEFINITION His-tagged arsenic repressor fused to superfolder GFP under the control of T7 promoter ACCESSION BBa_K1758203 VERSION BBa_K1758203.1 FEATURES Location/Qualifiers misc_feature 21..32 /label=BBa_B0034 misc_feature 1..32 /label=BBa_K525998 RBS 24..27 /label=RBS promoter 1..23 /label=T7 Promoter misc_feature 1123..1146 /label=His-Tag CDS 50..402 /label=arsR CDS 409..1122 /label=sfGFP misc_feature 1155..1234 /label=BBa_B0010 misc_feature 1243..1283 /label=BBa_B0012 ORIGIN 1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tgtcatttct 61 gttacccatc caattgttca aaattcttgc tgatgaaacc cgtctgggca tcgttttact 121 gctcagcgaa ctgggagagt tatgcgtctg cgatctctgc actgctctcg accagtcgca 181 gcccaagatc tcccgccacc tggcattgct gcgtgaaagc gggctattgc tggaccgcaa 241 gcaaggtaag tgggttcatt accgcttatc accgcatatt ccagcatggg cggcgaaaat 301 tattgatgag gcctggcgat gtgaacagga aaaggttcag gcgattgtcc gcaacctggc 361 tcgacaaaac tgttccgggg acagtaagaa catttgcagt taaccggcat gcgtaaaggc 421 gaagagctgt tcactggtgt cgtccctatt ctggtggaac tggatggtga tgtcaacggt 481 cataagtttt ccgtgcgtgg cgagggtgaa ggtgacgcaa ctaatggtaa actgacgctg 541 aagttcatct gtactactgg taaactgccg gtaccttggc cgactctggt aacgacgctg 601 acttatggtg ttcagtgctt tgctcgttat ccggaccata tgaagcagca tgacttcttc 661 aagtccgcca tgccggaagg ctatgtgcag gaacgcacga tttcctttaa ggatgacggc 721 acgtacaaaa cgcgtgcgga agtgaaattt gaaggcgata ccctggtaaa ccgcattgag 781 ctgaaaggca ttgactttaa agaagacggc aatatcctgg gccataagct ggaatacaat 841 tttaacagcc acaatgttta catcaccgcc gataaacaaa aaaatggcat taaagcgaat 901 tttaaaattc gccacaacgt ggaggatggc agcgtgcagc tggctgatca ctaccagcaa 961 aacactccaa tcggtgatgg tcctgttctg ctgccagaca atcactatct gagcacgcaa 1021 agcgttctgt ctaaagatcc gaacgagaaa cgcgatcata tggttctgct ggagttcgta 1081 accgcagcgg gcatcacgca tggtatggat gaactgtaca aacaccatca ccatcaccat 1141 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 1201 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 1261 gggtgggcct ttctgcgttt ata //