BBa_K1758203 1 BBa_K1758203 His-tagged arsenic repressor fused to superfolder GFP under the control of T7 promoter 2015-08-28T11:00:00Z 2015-09-18T05:53:38Z This is a composite part which enables a high protein expression and an easy purification of arsR fused to superfolding GFP (sfGFP). The green fluorescence of the culture is a sign for protein expression. Uses T7 promotor and a strong rbs (BBa_K525998), the arsenic repressor linked with superfolding GFP (sfGFP) with a His-Tag. By linking sfGFP we want to detect bound protein via fluorescence. false false _2180_ 26913 26913 9 false We clone arsR in T7-RBS-sfGFP and added a His-Tag via PCR and Gibson Assembly. false Team Bielefeld-CeBiTec 2015 annotation2440039 1 T7 Promoter range2440039 1 1 23 annotation2440036 1 BBa_B0034 range2440036 1 21 32 annotation2440038 1 RBS range2440038 1 24 27 annotation2440042 1 sfGFP range2440042 1 409 1122 annotation2440044 1 BBa_B0012 range2440044 1 1243 1283 annotation2440040 1 His-Tag range2440040 1 1123 1146 annotation2440041 1 arsR range2440041 1 50 402 annotation2440037 1 BBa_K525998 range2440037 1 1 32 annotation2440043 1 BBa_B0010 range2440043 1 1155 1234 BBa_K1758203_sequence 1 taatacgactcactatagggagatactagagaaagaggagaaatactagatgtcatttctgttacccatccaattgttcaaaattcttgctgatgaaacccgtctgggcatcgttttactgctcagcgaactgggagagttatgcgtctgcgatctctgcactgctctcgaccagtcgcagcccaagatctcccgccacctggcattgctgcgtgaaagcgggctattgctggaccgcaagcaaggtaagtgggttcattaccgcttatcaccgcatattccagcatgggcggcgaaaattattgatgaggcctggcgatgtgaacaggaaaaggttcaggcgattgtccgcaacctggctcgacaaaactgttccggggacagtaagaacatttgcagttaaccggcatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaacaccatcaccatcaccattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z