LOCUS       BBa_K1758204            1755 bp    DNA     linear       07-May-2024
DEFINITION  His-tagged blc repressor fused to superfolder GFP under the 
            control of the T7 promoter
ACCESSION   BBa_K1758204
VERSION     BBa_K1758204.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=T7 promoter
     RBS             24..27
                     /label=RBS
     misc_feature    1..32
                     /label=BBa_K525998
     misc_feature    21..32
                     /label=BBa_B0034
     misc_feature    1715..1755
                     /label=BBa_B0012
     misc_feature    1627..1706
                     /label=BBa_B0010
     CDS             50..874
                     /label=blcR
     CDS             881..1594
                     /label=sfGFP
     misc_feature    1595..1618
                     /label=His-Tag
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tgggccaaag
       61 gggccaagtg tgtcaaggaa ggtgcatggc tgaagatcaa caatcgtcgc aaatatcaga
      121 cactgtacca gcactcagac gcgccgtgcg tattctggat cttgtggcag gttccccgcg
      181 ggaccttaca gccgccgagc tgacacgcat tctggatttg cccaaaagca gcgcgcatgg
      241 cttgcttgcg gtgatgactg agcttgatct tctggcgcga tctgccgatg gaaccctgcg
      301 tattggaccc cactcgctca gatgggcaaa tggttttctg tcgcacctcg atatagtatc
      361 gacattcaac gaccatctcg cccagcgcca cgacctcgat ccctacacgg tgaccctcac
      421 cgtccgcgag ggtggcgaag tcgtttacat cggctgtcgc aactcggctc agccgcttgg
      481 acacacgttc cggatcggta tgcgtctgcc ggcgccattt acggccaccg ggaagattct
      541 cctgtccgat ctggggcctg gtgaattgag gatgctgttc tctcagtttc cacagcctct
      601 gacatcaagg agtgttgctg gcctttcgca gcttgaagag gaactggctc tgacgcgcgc
      661 tcgcggctac tccatcgacg atggtcagat ccgcgaaggt atgctttgca ttggggctgc
      721 gatacgcgat tactcgggag ccgcatctgc cggcattgca atcagtctga tccgcagcga
      781 agccagcgac gaaaaaatcg ctagccttgg tgaggagctt cgcaccactg ccaacgcgct
      841 ttctgaaaag cttgggtacc gatcgcagaa agacaccggc atgcgtaaag gcgaagagct
      901 gttcactggt gtcgtcccta ttctggtgga actggatggt gatgtcaacg gtcataagtt
      961 ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt aaactgacgc tgaagttcat
     1021 ctgtactact ggtaaactgc cggtaccttg gccgactctg gtaacgacgc tgacttatgg
     1081 tgttcagtgc tttgctcgtt atccggacca tatgaagcag catgacttct tcaagtccgc
     1141 catgccggaa ggctatgtgc aggaacgcac gatttccttt aaggatgacg gcacgtacaa
     1201 aacgcgtgcg gaagtgaaat ttgaaggcga taccctggta aaccgcattg agctgaaagg
     1261 cattgacttt aaagaagacg gcaatatcct gggccataag ctggaataca attttaacag
     1321 ccacaatgtt tacatcaccg ccgataaaca aaaaaatggc attaaagcga attttaaaat
     1381 tcgccacaac gtggaggatg gcagcgtgca gctggctgat cactaccagc aaaacactcc
     1441 aatcggtgat ggtcctgttc tgctgccaga caatcactat ctgagcacgc aaagcgttct
     1501 gtctaaagat ccgaacgaga aacgcgatca tatggttctg ctggagttcg taaccgcagc
     1561 gggcatcacg catggtatgg atgaactgta caaacaccat caccatcacc attaataata
     1621 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1681 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1741 ctttctgcgt ttata
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