BBa_K1758204 1 BBa_K1758204 His-tagged blc repressor fused to superfolder GFP under the control of the T7 promoter 2015-08-28T11:00:00Z 2015-09-18T07:21:49Z We clone blcR in T7-RBS-sfGFP and added a His-Tag via PCR and Gibson Assembly. Uses T7 promotor and a strong rbs (BBa_K525998), the blc repressor linked with superfolding GFP (sfGFP) with a His-Tag. By linking sfGFP we want to detect bound protein via fluorescence. false false _2180_ 26913 26913 9 false This is a composite part which enables a high protein expression and an easy purification of blcR fused to superfolding GFP (sfGFP). The green fluorescence of the culture is a sign for protein expression. false Team Bielefeld-CeBiTec 2015 annotation2440049 1 BBa_B0012 range2440049 1 1715 1755 annotation2440050 1 BBa_B0010 range2440050 1 1627 1706 annotation2440051 1 blcR range2440051 1 50 874 annotation2440052 1 sfGFP range2440052 1 881 1594 annotation2440047 1 BBa_K525998 range2440047 1 1 32 annotation2440053 1 His-Tag range2440053 1 1595 1618 annotation2440045 1 T7 promoter range2440045 1 1 23 annotation2440046 1 RBS range2440046 1 24 27 annotation2440048 1 BBa_B0034 range2440048 1 21 32 BBa_K1758204_sequence 1 taatacgactcactatagggagatactagagaaagaggagaaatactagatgggccaaaggggccaagtgtgtcaaggaaggtgcatggctgaagatcaacaatcgtcgcaaatatcagacactgtaccagcactcagacgcgccgtgcgtattctggatcttgtggcaggttccccgcgggaccttacagccgccgagctgacacgcattctggatttgcccaaaagcagcgcgcatggcttgcttgcggtgatgactgagcttgatcttctggcgcgatctgccgatggaaccctgcgtattggaccccactcgctcagatgggcaaatggttttctgtcgcacctcgatatagtatcgacattcaacgaccatctcgcccagcgccacgacctcgatccctacacggtgaccctcaccgtccgcgagggtggcgaagtcgtttacatcggctgtcgcaactcggctcagccgcttggacacacgttccggatcggtatgcgtctgccggcgccatttacggccaccgggaagattctcctgtccgatctggggcctggtgaattgaggatgctgttctctcagtttccacagcctctgacatcaaggagtgttgctggcctttcgcagcttgaagaggaactggctctgacgcgcgctcgcggctactccatcgacgatggtcagatccgcgaaggtatgctttgcattggggctgcgatacgcgattactcgggagccgcatctgccggcattgcaatcagtctgatccgcagcgaagccagcgacgaaaaaatcgctagccttggtgaggagcttcgcaccactgccaacgcgctttctgaaaagcttgggtaccgatcgcagaaagacaccggcatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaacaccatcaccatcaccattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z