LOCUS       BBa_K1758300            1006 bp    DNA     linear       01-Jun-2024
DEFINITION  sfGFP controlled by T7 promoter and arsenic operator
ACCESSION   BBa_K1758300
VERSION     BBa_K1758300.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=T7 promoter
     misc_feature    1..23
                     /label=BBa_I719005
     CDS             150..866
                     /label=sfGFP
     RBS             137..144
                     /label=BBa_B0034
     misc_feature    878..957
                     /label=BBa_B0010
     misc_feature    966..1006
                     /label=BBa_B0012
     misc_feature    67..85
                     /label=ArsR binding site
     misc_feature    104..149
                     /label=5'-UTR
ORIGIN
        1 taatacgact cactataggg agatactaga gccaactcaa aattcacacc tattaccttc
       61 ctctgcactt acacattcgt taagtcatat atgtttttga cttaataatt ttgtttaact
      121 ttaaaaaaaa aaaagaagga gatatacata tgcgtaaagg cgaagagctg ttcactggtg
      181 tcgtccctat tctggtggaa ctggatggtg atgtcaacgg tcataagttt tccgtgcgtg
      241 gcgagggtga aggtgacgca actaatggta aactgacgct gaagttcatc tgtactactg
      301 gtaaactgcc ggtaccttgg ccgactctgg taacgacgct gacttatggt gttcagtgct
      361 ttgctcgtta tccggaccat atgaagcagc atgacttctt caagtccgcc atgccggaag
      421 gctatgtgca ggaacgcacg atttccttta aggatgacgg cacgtacaaa acgcgtgcgg
      481 aagtgaaatt tgaaggcgat accctggtaa accgcattga gctgaaaggc attgacttta
      541 aagaagacgg caatatcctg ggccataagc tggaatacaa ttttaacagc cacaatgttt
      601 acatcaccgc cgataaacaa aaaaatggca ttaaagcgaa ttttaaaatt cgccacaacg
      661 tggaggatgg cagcgtgcag ctggctgatc actaccagca aaacactcca atcggtgatg
      721 gtcctgttct gctgccagac aatcactatc tgagcacgca aagcgttctg tctaaagatc
      781 cgaacgagaa acgcgatcat atggttctgc tggagttcgt aaccgcagcg ggcatcacgc
      841 atggtatgga tgaactgtac aaatgatgat actagagcca ggcatcaaat aaaacgaaag
      901 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      961 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//