BBa_K1758300 1 BBa_K1758300 sfGFP controlled by T7 promoter and arsenic operator 2015-08-21T11:00:00Z 2015-09-18T04:46:17Z Based on three parts from the distribution. This part consists of sfGFP with a translation enhancing 5'-UTR under the control of the T7 promoter and arsenic operator. In combination with the E. coli arsenic repressor arsR, this part can be used as an arsenic biosensor. false false _2180_ 27935 26587 9 false This part was created by inserting the T7 promoter BBa_I719005 upstream of BBa_J33201 via 3A assembly. Subsequently, Gibson assembly was performed to replace arsR by sfGFP and introduce the UTR. The followoing primers and templates were used: Fragment 1: UTR_arsO_rev: ccttcttttttttttttaaagttaaacaaaattatTAAGTCAAAAACATATATGACTTAACG Cm_fwd: CGGCATCAGCACCTTGTC Template: pSB1C3-pT7-arsR Fragment 2: UTR_fwd: GTTTAACTTTAAAAAAAAAAAAGAAGGAGATATACATATGCGTAAAGGCGAAGAGCTGTTCAC Cm_rev: TATACGCAAGGCGACAAG Template: BBa_I746909 false Team Bielefeld-CeBiTec 2015 annotation2436868 1 BBa_B0034 range2436868 1 137 144 annotation2436865 1 T7 promoter range2436865 1 1 23 annotation2436869 1 BBa_B0010 range2436869 1 878 957 annotation2436870 1 BBa_B0012 range2436870 1 966 1006 annotation2436871 1 ArsR binding site range2436871 1 67 85 annotation2436866 1 BBa_I719005 range2436866 1 1 23 annotation2436872 1 5'-UTR range2436872 1 104 149 annotation2436867 1 sfGFP range2436867 1 150 866 BBa_K1758300_sequence 1 taatacgactcactatagggagatactagagccaactcaaaattcacacctattaccttcctctgcacttacacattcgttaagtcatatatgtttttgacttaataattttgtttaactttaaaaaaaaaaaagaaggagatatacatatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z