LOCUS BBa_K1758313 2081 bp DNA linear 25-Nov-2024 DEFINITION Chromium repressor under control of constitutive promoter and strong RBS,chromium responsive promote ACCESSION BBa_K1758313 VERSION BBa_K1758313.1 FEATURES Location/Qualifiers promoter 945..1000 /label=K608002 CDS 1..944 /label=ChrB misc_feature 1..1000 /label=BBa_K1758310 promoter 1009..1172 /label=Cr-promoter misc_feature 1179..1224 /label=5-UTR CDS 1225..1944 /label=sfGFP misc_feature 1953..2032 /label=T1 misc_feature 2041..2081 /label=T7-term misc_feature 1009..2081 /label=BBa_K1758312 ORIGIN 1 tcaaacggtc agagtgcctt tttcaaaaga agcgagcaga ccatcaaaca cagtgctagc 61 gatggctaat aactgatcgt catgatccac agtctcacgc agaccggcca gaacggactc 121 gataccggtg gcctccggtg gttgaatgcc acccacgtcc agatagtgca cgaccaggcc 181 aatacgggta atcgccggtt gttccaggcc aaaggacgcg gcaagaactt cgaaggtcac 241 gcgtgaccct acgtgtgaaa acgttgcacc atcaaagtcg aagcccagcg catccggggg 301 gcaatcggcc ggggttgcga gccagagaat acgtgcttga gggtcgatga aacggcggat 361 cagccacgct gaggcgaggc gatccaccca aggacgagca cgggttgccc aggtgcgtgc 421 ctggtaatcc aagcggtcaa gacgggtgat cgtgccttca accgcgtgcg gttcgtctgg 481 cgataaggtg cgggcacagg cctgttccag ttcacacaga gccgagtcgg cctgacgctg 541 cgcttcaccc ggatagaagt caatttccac cagggttgta aagcttttgc gaagcttgcg 601 cacctgacga agaacgtcct gcactgtatc taaagtcagt gtctgacgca ggtggtggac 661 atctaccaat aaagccgcaa aatcattact gcggtcaaat aacgcgacaa agttaacgcc 721 ttccgggtct tccatgcgca gcacgtgcgc tacgccgccg ccctcacgaa catcggacgc 781 cagattatcc aggaccgcac gacattcatc gcgatccggc atcagatata ccccatcacg 841 caggaccgct gcgccggagg ccttcagcgc acgccacgta cgttggcgga ctgtcgcgtt 901 ttctgtgggt aagctgagga tgggtgacag taagttcatc tagtatttct cctctttctc 961 tagtagctag cacaatacct aggactgagc tagctgtcaa tactagagga ttgcttattc 1021 ctattgccat ttgcttttat tttgcaatac gttttagcaa cgcacaacac cttatgggtg 1081 tgctgcttcc attgtgattg cgcaaatgtc cgtttttgca atctactcaa gactttattt 1141 cgtagatctt atctcattat tgtagtaaca tctacgacaa taattttgtt taactttaaa 1201 aaaaaaaaag aaggagaata atctatgcgt aaaggcgaag agctgttcac tggtgtcgtc 1261 cctattctgg tggaactgga tggtgatgtc aacggtcata agttttccgt gcgtggcgag 1321 ggtgaaggtg acgcaactaa tggtaaactg acgctgaagt tcatctgtac tactggtaaa 1381 ctgccggtac cttggccgac tctggtaacg acgctgactt atggtgttca gtgctttgct 1441 cgttatccgg accatatgaa gcagcatgac ttcttcaagt ccgccatgcc ggaaggctat 1501 gtgcaggaac gcacgatttc ctttaaggat gacggcacgt acaaaacgcg tgcggaagtg 1561 aaatttgaag gcgataccct ggtaaaccgc attgagctga aaggcattga ctttaaagaa 1621 gacggcaata tcctgggcca taagctggaa tacaatttta acagccacaa tgtttacatc 1681 accgccgata aacaaaaaaa tggcattaaa gcgaatttta aaattcgcca caacgtggag 1741 gatggcagcg tgcagctggc tgatcactac cagcaaaaca ctccaatcgg tgatggtcct 1801 gttctgctgc cagacaatca ctatctgagc acgcaaagcg ttctgtctaa agatccgaac 1861 gagaaacgcg atcatatggt tctgctggag ttcgtaaccg cagcgggcat cacgcatggt 1921 atggatgaac tgtacaaatg atgatactag agccaggcat caaataaaac gaaaggctca 1981 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 2041 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //