LOCUS       BBa_K1758333            1484 bp    DNA     linear       25-Nov-2024
DEFINITION  Lead repressor under control of constitutive promoter and strong 
            RBS and lead responsive promoter wi
ACCESSION   BBa_K1758333
VERSION     BBa_K1758333.1  
FEATURES             Location/Qualifiers
     promoter        444..499
                     /label=K608002
     CDS             1..438
                     /label=PbrR
     misc_feature    1..499
                     /label=BBa_K1758330
     promoter        508..575
                     /label=PbrA
     misc_feature    582..627
                     /label=5??UTR
     CDS             628..1347
                     /label=sfGFP
     misc_feature    1356..1435
                     /label=T1
     misc_feature    1444..1484
                     /label=T7-term
     misc_feature    508..1484
                     /label=BBa_K1758332
ORIGIN
        1 ttaatcggac gggtgcgcgg tggtgccacg ggtatcgcaa acgcagtcac taaggccttg
       61 taagatgccg caggattggg ccggacgcgc gcctgaacaa gcctcacgca gttcaaccaa
      121 gtgatgcttc agttcaagga gggcaccaat acggctttca acctggcgaa tgtgttcatc
      181 gagcagcata ttcacttcac cgcaatcctg gtcgggacgt ttccgatacg acaacagggt
      241 acgaacgtca ctcagcggca tatcgagact acgacaatga cgaataaatt gaaggcgctc
      301 aacatgttct tcgccataaa gacgaaaatt accccgactg cggcccggcg gcggaagcaa
      361 cccttcttgt tcgtaaaagc ggatggtcac caccggacac gctgtgcgtt tcgccagttc
      421 cccgatctga atgttcatct agtatttctc ctctttctct agtagctagc acaataccta
      481 ggactgagct agctgtcaat actagagtgg gagatgtctt gactctatag taactagagg
      541 gtgttaaatc ggcaacgcga gaaaaagagg agaaatacta gaataatttt gtttaacttt
      601 aaaaaaaaaa aagaaggaga ataatctatg cgtaaaggcg aagagctgtt cactggtgtc
      661 gtccctattc tggtggaact ggatggtgat gtcaacggtc ataagttttc cgtgcgtggc
      721 gagggtgaag gtgacgcaac taatggtaaa ctgacgctga agttcatctg tactactggt
      781 aaactgccgg taccttggcc gactctggta acgacgctga cttatggtgt tcagtgcttt
      841 gctcgttatc cggaccatat gaagcagcat gacttcttca agtccgccat gccggaaggc
      901 tatgtgcagg aacgcacgat ttcctttaag gatgacggca cgtacaaaac gcgtgcggaa
      961 gtgaaatttg aaggcgatac cctggtaaac cgcattgagc tgaaaggcat tgactttaaa
     1021 gaagacggca atatcctggg ccataagctg gaatacaatt ttaacagcca caatgtttac
     1081 atcaccgccg ataaacaaaa aaatggcatt aaagcgaatt ttaaaattcg ccacaacgtg
     1141 gaggatggca gcgtgcagct ggctgatcac taccagcaaa acactccaat cggtgatggt
     1201 cctgttctgc tgccagacaa tcactatctg agcacgcaaa gcgttctgtc taaagatccg
     1261 aacgagaaac gcgatcatat ggttctgctg gagttcgtaa ccgcagcggg catcacgcat
     1321 ggtatggatg aactgtacaa atgatgatac tagagccagg catcaaataa aacgaaaggc
     1381 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1441 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
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