LOCUS       BBa_K1758353            1353 bp    DNA     linear       12-May-2024
DEFINITION  Nickel repressor under control of constitutive promoter and strong 
            RBS and Nickel responsive promote
ACCESSION   BBa_K1758353
VERSION     BBa_K1758353.1  
FEATURES             Location/Qualifiers
     promoter        279..334
                     /label=K608002
     CDS             1..272
                     /label=rcnR
     misc_feature    1..334
                     /label=BBa_K1758350
     promoter        341..444
                     /label=RcnA
     misc_feature    451..496
                     /label=5??UTR
     CDS             497..1216
                     /label=sfGFP
     misc_feature    1225..1304
                     /label=T1
     misc_feature    1313..1353
                     /label=T7-term
     misc_feature    341..1353
                     /label=BBa_K1758352
ORIGIN
        1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaatacta
       61 gatgtctcat accatccgtg ataaacagaa gttaaaagcc cgcgcctcta aaatccaggg
      121 tcaagtcgtc gcattaaaga agatgctgga tgaaccacac gaatgcgcag cggtgttgca
      181 acaaatcgct gcgattcgcg gagccgttaa tggtttaatg cgtgaggtca tcaaaggcca
      241 tcttaccgaa cacattgtcc accaagggga tgaactgaaa cgtgaggagg acctggacgt
      301 ggtcttgaaa gtccttgatt cgtacatcaa atgatactag atgagacatg gcaacacctg
      361 gttaacaaga atatgaaaaa tcatagcact attaatctac tggggggtag tatcaggtac
      421 tgggggggag taaaagagga gaaatactag aataattttg tttaacttta aaaaaaaaaa
      481 agaaggagaa taatctatgc gtaaaggcga agagctgttc actggtgtcg tccctattct
      541 ggtggaactg gatggtgatg tcaacggtca taagttttcc gtgcgtggcg agggtgaagg
      601 tgacgcaact aatggtaaac tgacgctgaa gttcatctgt actactggta aactgccggt
      661 accttggccg actctggtaa cgacgctgac ttatggtgtt cagtgctttg ctcgttatcc
      721 ggaccatatg aagcagcatg acttcttcaa gtccgccatg ccggaaggct atgtgcagga
      781 acgcacgatt tcctttaagg atgacggcac gtacaaaacg cgtgcggaag tgaaatttga
      841 aggcgatacc ctggtaaacc gcattgagct gaaaggcatt gactttaaag aagacggcaa
      901 tatcctgggc cataagctgg aatacaattt taacagccac aatgtttaca tcaccgccga
      961 taaacaaaaa aatggcatta aagcgaattt taaaattcgc cacaacgtgg aggatggcag
     1021 cgtgcagctg gctgatcact accagcaaaa cactccaatc ggtgatggtc ctgttctgct
     1081 gccagacaat cactatctga gcacgcaaag cgttctgtct aaagatccga acgagaaacg
     1141 cgatcatatg gttctgctgg agttcgtaac cgcagcgggc atcacgcatg gtatggatga
     1201 actgtacaaa tgatgatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1261 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1321 gctcaccttc gggtgggcct ttctgcgttt ata
//