##gff-version 3
##sequence-region BBa_K1758361 1555 2418
BBa_K1758361	.	engineered_region	1555	2418	.	+	0	ID=BBa_K516030;Name=BBa_K516030
BBa_K1758361	.	ribosome_entry_site	1555	1569	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K516030
BBa_K1758361	.	ribosome_entry_site	1555	1569	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1758361	.	engineered_region	1555	1569	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1758361	.	ribosome_entry_site	1562	1566	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1758361	.	CDS	1576	2281	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K516030
BBa_K1758361	.	engineered_tag	2257	2281	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1758361	.	CDS	1576	2250	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1758361	.	terminator	2290	2418	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K516030
BBa_K1758361	.	terminator	2290	2369	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1758361	.	engineered_region	2290	2369	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1758361	.	stem_loop	2301	2344	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1758361	.	terminator	2378	2418	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1758361	.	stem_loop	2385	2404	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1758361	.	engineered_region	2378	2418	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1758361	.	polyA_site	2405	2418	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1758361	.	stop_codon	2411	2411	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_K1758361
tcaatcacctgtgactgggatttttttatggccgtacactgctttaaacaagcgctgaacaccttcctgaatatcttct
tctttgagacgcgcaaaaccgatgatcagggctggacgcccagtctggcgtttattttctttcagattgaagcgcgaca
tgccaaaaatttccaattgcaaaccggccgcatggctcagaatgtcctgttctgtacgacgggtgtcaaattccgtcac
gaagtgcaggcccgcgtttgcccctttaaccgtcacttcgccagagaactcggcctccagggccgtgatcaggcgttca
cgtttttctttgtaatgctgtttcattttcttaatgtgcttctgatattcacctgattcgataaattcttgcagtgtaa
gctgcgtaagactgctacaggtttgcaggtcatagccacgctgtttatacgcgcgcaacaattccggcggcagaaccat
gtaagaaatacgaagacccggaagcaaacttttagagaaggtacccatgtagataacattctgaaagcgatccaggctc
tgaagcgccgggatggagtccacgtcgtaagtgaactcactgtcgtagtcatcttcaatgatataacggcgcggttcct
ctgccgcccaattcaggagctgaatgcgacgagaaacgggcataatcgtaccagatggaaactggtggctaggggttgt
cacaagcacgtcaggctgttggcgcgtaatctctgcgatggacatgcccttttcatctaacatgatcgttttcacttgt
ttaccggcgtttttcagaagctgatacatgcggcgataaccaggttcttccattgcgtatactgcctctttcggcagca
gttcagttaataactgcatcagcacctgagtcccggcgccaatgatcatctgttccggacggcatttgacaccgcgggt
aagtgaaattaagcgggtgatggcggcacgtacttcgtagatgccctgcggatggctcatatctcctaaagtacgatac
gaacggctcgcggccttttgttcacagcggaaccacgatttaataggaaagtgatcggtatccgaagacatatgggaga
agctgatccaatcggactgatcaatatggatttctttcagatcgtccgggagggcgaacggaggatgttcctctgcgct
gaacatgtccagttcctccacaaagaatcctttgcgctcaatcgcgtacaggtagccctctgcaagcagctgctggtat
gcgctattcacgctattgactgacactttgaggttttcggccagttcacgtttactcggaactttggaatgtggcagca
agttgcgggaaagaatttcttttttcagtttctgatagatctgttggtagatatagtccgcctgttccgagcgatccag
agtaatcgtaatgtccattttgtagtttctccttctgataccatcaaaaagttataattggtacttttcatcataccaa
agagaagtcagaatgataagaaaataccgacagggggatattcactactagagattaaagaggagaaatactagatggc
ttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaa
atcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgc
cgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccgga
ctacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgtt
acccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtc
cggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtga
aatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccg
gttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaac
agtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttata