LOCUS       BBa_K1758376             966 bp    DNA     linear       25-Dec-2024
DEFINITION  GHB / GBL biosensor - reporter part
ACCESSION   BBa_K1758376
VERSION     BBa_K1758376.1  
FEATURES             Location/Qualifiers
     promoter        1..22
                     /label=T7 promoter
     misc_feature    23..63
                     /label=Blc operator
     misc_feature    64..109
                     /label=5' -UTR
     misc_feature    64..109
                     /label=K1758100
     misc_feature    64..966
                     /label=K1758101
     CDS             110..829
                     /label=sfGFP
     misc_feature    838..917
                     /label=B0010
     misc_feature    926..966
                     /label=B0012
     misc_feature    691..691
                     /label=silent mutation
ORIGIN
        1 taatacgact cactataggg agttcactct aatgattcaa gttcaattag ttgaactcta
       61 atgaataatt ttgtttaact ttaaaaaaaa aaaagaagga gaataatcta tgcgtaaagg
      121 cgaagagctg ttcactggtg tcgtccctat tctggtggaa ctggatggtg atgtcaacgg
      181 tcataagttt tccgtgcgtg gcgagggtga aggtgacgca actaatggta aactgacgct
      241 gaagttcatc tgtactactg gtaaactgcc ggtaccttgg ccgactctgg taacgacgct
      301 gacttatggt gttcagtgct ttgctcgtta tccggaccat atgaagcagc atgacttctt
      361 caagtccgcc atgccggaag gctatgtgca ggaacgcacg atttccttta aggatgacgg
      421 cacgtacaaa acgcgtgcgg aagtgaaatt tgaaggcgat accctggtaa accgcattga
      481 gctgaaaggc attgacttta aagaagacgg caatatcctg ggccataagc tggaatacaa
      541 ttttaacagc cacaatgttt acatcaccgc cgataaacaa aaaaatggca ttaaagcgaa
      601 ttttaaaatt cgccacaacg tggaggatgg cagcgtgcag ctggctgatc actaccagca
      661 aaacactcca atcggtgatg gtcctgttct actgccagac aatcactatc tgagcacgca
      721 aagcgttctg tctaaagatc cgaacgagaa acgcgatcat atggttctgc tggagttcgt
      781 aaccgcagcg ggcatcacgc atggtatgga tgaactgtac aaatgatgat actagagcca
      841 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      901 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
      961 tttata
//