BBa_K1758376 1 BBa_K1758376 GHB / GBL biosensor - reporter part 2015-08-31T11:00:00Z 2015-09-19T01:22:36Z This part was assembled via PCR with Gibson primers on [http://parts.igem.org/Part:BBa_K1758102 BBa_K1758102] This part contains all features from [http://parts.igem.org/Part:BBa_K1758102 BBa_K1758102] plus the blc-operator site described in [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083662/ Pan et al. 2013]. BlcR (see http://parts.igem.org/Part:BBa_K1758370 K1758370]) can bind to the operator site. false false _2180_ 26563 26563 9 false --- false Team Bielefeld-CeBiTec 2015 annotation2441684 1 B0012 range2441684 1 926 966 annotation2441679 1 5' -UTR range2441679 1 64 109 annotation2441678 1 Blc operator range2441678 1 23 63 annotation2441682 1 sfGFP range2441682 1 110 829 annotation2441694 1 silent mutation range2441694 1 691 691 annotation2441681 1 K1758101 range2441681 1 64 966 annotation2441677 1 T7 promoter range2441677 1 1 22 annotation2441680 1 K1758100 range2441680 1 64 109 annotation2441683 1 B0010 range2441683 1 838 917 BBa_K1758370 1 BBa_K1758370 BlcR, repressor of blcABC operon, with promoter and RBS 2015-08-26T11:00:00Z 2015-09-19T01:56:27Z This sequence comes from megaplasmid pATC58 from ''Agrobacterium tumefaciens'' strain C58.1 BlcR, a gene of ''Agrobacterium tumefaciens'', represses the ''blcABC''-Operon which enables the bacterium to grow on gamma-butyrolactone as carbon and energy source ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083662/ Pan et al. 2013]). false false _2180_ 26563 26563 9 false BlcR coding sequence was cloned into pSB1C3 with Gibson primers false Team Bielefeld-CeBiTec 2015 annotation2438823 1 BlcR range2438823 1 62 889 annotation2438822 1 K608002 range2438822 1 1 55 BBa_K1758377 1 BBa_K1758377 Biosensor device for detection of GHB and GBL 2015-09-09T11:00:00Z 2015-09-18T07:41:49Z Biobrick assembly This part serves as a generator of sfGFP when induced. false false _2180_ 26563 26563 9 true --- false Team Bielefeld-CeBiTec 2015 component2448545 1 BBa_K1758376 component2448548 1 BBa_K1758370 annotation2448548 1 BBa_K1758370 range2448548 1 975 1863 annotation2448545 1 BBa_K1758376 range2448545 1 1 966 BBa_K1758376_sequence 1 taatacgactcactatagggagttcactctaatgattcaagttcaattagttgaactctaatgaataattttgtttaactttaaaaaaaaaaaagaaggagaataatctatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctactgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1758377_sequence 1 taatacgactcactatagggagttcactctaatgattcaagttcaattagttgaactctaatgaataattttgtttaactttaaaaaaaaaaaagaaggagaataatctatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctactgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaatactagatgggccaaaggggccaagtgtgtcaaggaaggtgcatggctgaagatcaacaatcgtcgcaaatatcagacactgtaccagcactcagacgcgccgtgcgtattctggatcttgtggcaggttccccgcgggaccttacagccgccgagctgacacgcattctggatttgcccaaaagcagcgcgcatggcttgcttgcggtgatgactgagcttgatcttctggcgcgatctgccgatggaaccctgcgtattggaccccactcgctcagatgggcaaatggttttctgtcgcacctcgatatagtatcgacattcaacgaccatctcgcccagcgccacgacctcgatccctacacggtgaccctcaccgtccgcgagggtggcgaagtcgtttacatcggctgtcgcaactcggctcagccgcttggacacacgttccggatcggtatgcgtctgccggcgccatttacggccaccgggaagattctcctgtccgatctggggcctggtgaattgaggatgctgttctctcagtttccacagcctctgacatcaaggagtgttgctggcctttcgcagcttgaagaggaactggctctgacgcgcgctcgcggctactccatcgacgatggtcagatccgcgaaggtatgctttgcattggggctgcgatacgcgattactcgggagccgcatctgccggcattgcaatcagtctgatccgcagcgaagccagcgacgaaaaaatcgctagccttggtgaggagcttcgcaccactgccaacgcgctttctgaaaagcttgggtaccgatcgcagaaagactaa BBa_K1758370_sequence 1 ttgacagctagctcagtcctaggtattgtgctagctactagagaaagaggagaaatactagatgggccaaaggggccaagtgtgtcaaggaaggtgcatggctgaagatcaacaatcgtcgcaaatatcagacactgtaccagcactcagacgcgccgtgcgtattctggatcttgtggcaggttccccgcgggaccttacagccgccgagctgacacgcattctggatttgcccaaaagcagcgcgcatggcttgcttgcggtgatgactgagcttgatcttctggcgcgatctgccgatggaaccctgcgtattggaccccactcgctcagatgggcaaatggttttctgtcgcacctcgatatagtatcgacattcaacgaccatctcgcccagcgccacgacctcgatccctacacggtgaccctcaccgtccgcgagggtggcgaagtcgtttacatcggctgtcgcaactcggctcagccgcttggacacacgttccggatcggtatgcgtctgccggcgccatttacggccaccgggaagattctcctgtccgatctggggcctggtgaattgaggatgctgttctctcagtttccacagcctctgacatcaaggagtgttgctggcctttcgcagcttgaagaggaactggctctgacgcgcgctcgcggctactccatcgacgatggtcagatccgcgaaggtatgctttgcattggggctgcgatacgcgattactcgggagccgcatctgccggcattgcaatcagtctgatccgcagcgaagccagcgacgaaaaaatcgctagccttggtgaggagcttcgcaccactgccaacgcgctttctgaaaagcttgggtaccgatcgcagaaagactaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z