BBa_K1758390 1 BBa_K1758390 His-tagged E. coli tryptophanase under control of the T7 promoter 2015-08-21T11:00:00Z 2015-09-18T05:00:27Z This part was obtained by gene synthesis. This part can be used for expression and purification of the E. coli tryptophanase. A tryptophanase catalyzes the reaction of L-tryptophan and water to indole, pyruvate, and NH3. false false _2180_ 27935 26587 9 false This part was obtained as linear gene synthesis and cloned into pSB1C3 via Gibson Assembly. false Team Bielefeld-CeBiTec 2015 annotation2436880 1 tryptophanase range2436880 1 41 1453 annotation2436881 1 T7 promoter + RBS range2436881 1 1 32 annotation2436877 1 his-tag range2436877 1 1454 1471 annotation2436878 1 stop range2436878 1 1472 1474 BBa_K1758390_sequence 1 taatacgactcactatagggaaagaggagaaatactagagatggagaatttcaaacacttaccggagcccttccgcattcgcgtcatcgagccggtaaagcgtacaacgcgtgcgtatcgtgaagaagcgattatcaaaagtggaatgaacccatttctgctggatagcgaagatgtgtttatcgatcttctgaccgatagtggcaccggcgcggttacacaatcaatgcaggctgcgatgatgcgcggcgatgaagcttactcgggctctcgtagctattacgccttagcagagagcgtgaaaaatatcttcggctaccaatataccattccaacccatcagggacgtggtgccgagcaaatttacatcccagtgctgattaaaaaacgcgaacaggaaaaaggtttagatcgctccaaaatggttgccttctcaaactattttttcgacacaacacaggggcactcccagatcaacggttgcacggtgcgcaacgtctatattaaagaggcgtttgatacgggcgtccgctatgattttaaaggcaatttcgatttggaaggcctggagcgtggcatcgaggaagtgggcccgaataatgttccatatattgtcgcaaccattaccagcaacagtgccggcggtcaacctgttagcctggcgaatctgaaggccatgtatagtatcgccaagaaatatgacatcccggtggtgatggatagtgcgcgtttcgcagagaatgcctacttcatcaaacagcgcgaagctgaatacaaggattggacaattgaacaaatcacgcgtgaaacttacaaatacgcggacatgctcgcgatgtcagcgaaaaaggatgcaatggtccctatgggtggtctgctgtgcatgaaagatgacagttttttcgacgtttatacagaatgccgtacactgtgcgttgtgcaggaaggtttccctacatatggtggtctggaaggaggtgcaatggaacgtctggcagtaggcctgtatgacggcatgaaccttgactggctggcctatcgtattgcccaggtccagtatctggtcgacggactggaagaaatcggcgtagtctgccagcaggcgggcggccatgcggcctttgttgacgctggcaaacttttgcctcatatcccggcagatcagttccccgctcaggccttagcgtgcgagctgtataaagttgcagggatccgtgcggtcgaaatcggctctttcttgttgggacgtgatccgaaaaccggtaaacaattgccatgcccggctgaactgctgcgcttgaccattccacgtgccacctatacgcaaacgcacatggattttattatcgaagctttcaaacatgtgaaagaaaatgcggcgaacattaaaggcctgacttttacatatgaaccgaaagtactgcgtcactttaccgccaaactgaaagaagttcatcatcaccatcaccactaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z