LOCUS       BBa_K176002              955 bp    DNA     linear       26-Jun-2024
DEFINITION  pLux/Tet(K176000)(LuxR+,TetR-)->RBS+GFP+T
ACCESSION   BBa_K176002
VERSION     BBa_K176002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..53
                     /label=R0062
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    54..72
                     /label=tetR
     misc_feature    53..53
                     /label=+1
     misc_feature    1..20
                     /label=luxR/HSL
     promoter        1..72
                     /label=BBa_K176000
     misc_feature    85..88
                     /label=conserved
     RBS             81..92
                     /label=BBa_B0034
     CDS             99..818
                     /label=GFP protein
     CDS             99..818
                     /label=BBa_E0040
     stem_loop       838..881
                     /label=stem_loop
     misc_feature    827..906
                     /label=BBa_B0010
     terminator      827..906
                     /label=BBa_B0010
     stem_loop       922..941
                     /label=T7 TE
     misc_feature    948..948
                     /label=stop
     polyA_site      942..955
                     /label=polya
     misc_feature    915..955
                     /label=BBa_B0012
     terminator      915..955
                     /label=BBa_B0012
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga atatccctat
       61 cagtgataga gatactagag aaagaggaga aatactagat gcgtaaagga gaagaacttt
      121 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
      181 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt
      241 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg
      301 ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca
      361 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga
      421 cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta
      481 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac
      541 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta
      601 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa
      661 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt
      721 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg
      781 ggattacaca tggcatggat gaactataca aataataata ctagagccag gcatcaaata
      841 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
      901 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//