LOCUS       BBa_K176007             3024 bp    DNA     linear       29-Jun-2024
DEFINITION  pSB1A3 with the suffix of J61002 (mRFP)
ACCESSION   BBa_K176007
VERSION     BBa_K176007.1  
FEATURES             Location/Qualifiers
     misc_feature    9..20
                     /label=B0034
     misc_feature    2..7
                     /label=SpeI
     RBS             9..20
                     /label=RBS
     CDS             27..707
                     /label=mRFP
     misc_feature    741..869
                     /label=B0015
     stem_loop       752..795
                     /label=rrnB terminator
     misc_feature    878..885
                     /label=NotI
     misc_feature    885..890
                     /label=PstI
     misc_feature    1..890
                     /label=a part of J61002
     misc_feature    878..3026
                     /label=a part of pSB1A3
     misc_feature    3005..3026
                     /label=prefix
     misc_feature    902..903
                     /label=deletion of ac in pSB1A3
     misc_feature    1149..1149
                     /label=t->c in pSB1A3
     misc_feature    1584..1584
                     /label=g->a in pSB1A3
ORIGIN
        1 tactagtgaa agaggagaaa tactagatgg cttcctccga agacgttatc aaagagttca
       61 tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg
      121 aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg
      181 gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt
      241 acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca
      301 aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct
      361 ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg
      421 acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc
      481 cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc
      541 actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg
      601 cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg
      661 aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct
      721 agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
      781 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
      841 accttcgggt gggcctttct gcgtttatat actagaagcg gccgctgcag tccggcaaaa
      901 aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt
      961 atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     1021 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     1081 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1141 tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1201 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1261 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1321 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1381 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1441 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1501 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1561 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1621 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1681 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1741 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1801 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     1861 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     1921 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1981 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     2041 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     2101 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     2161 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     2221 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     2281 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     2341 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     2401 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     2461 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     2521 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     2581 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     2641 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     2701 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     2761 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     2821 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     2881 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     2941 cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc
     3001 tggaattcgc ggccgcttct agag
//