LOCUS       BBa_K176014              918 bp    DNA     linear       27-Dec-2024
DEFINITION  pCon 0.15(K176008)->RBS+GFP+T
ACCESSION   BBa_K176014
VERSION     BBa_K176014.1  
FEATURES             Location/Qualifiers
     promoter        1..6
                     /label=-35
     promoter        24..29
                     /label=-10
     misc_feature    17..17
                     /label=c in J23115
     misc_feature    21..21
                     /label=t in J23115
     misc_feature    7..12
                     /label=NheI
     misc_feature    30..35
                     /label=NheI
     misc_feature    18..23
                     /label=AvrII
     promoter        1..35
                     /label=BBa_K176008
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..781
                     /label=GFP protein
     CDS             62..781
                     /label=BBa_E0040
     stem_loop       801..844
                     /label=stem_loop
     misc_feature    790..869
                     /label=BBa_B0010
     terminator      790..869
                     /label=BBa_B0010
     stem_loop       885..904
                     /label=T7 TE
     misc_feature    911..911
                     /label=stop
     polyA_site      905..918
                     /label=polya
     misc_feature    878..918
                     /label=BBa_B0012
     terminator      878..918
                     /label=BBa_B0012
ORIGIN
        1 tttatagcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta
       61 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      121 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      181 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      241 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
      301 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      361 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      421 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      481 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
      541 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      601 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      661 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
      721 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
      781 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      841 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      901 ggcctttctg cgtttata
//