LOCUS BBa_K176022 841 bp DNA linear 15-Nov-2024 DEFINITION pCon 0.15(K176008)->RBS+luxI-LVA+T ACCESSION BBa_K176022 VERSION BBa_K176022.1 FEATURES Location/Qualifiers promoter 1..6 /label=-35 promoter 24..29 /label=-10 misc_feature 17..17 /label=c in J23115 misc_feature 21..21 /label=t in J23115 misc_feature 7..12 /label=NheI misc_feature 30..35 /label=NheI misc_feature 18..23 /label=AvrII promoter 1..35 /label=BBa_K176008 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 641..672 /label=LVA CDS 62..640 /label=luxI misc_feature 62..679 /label=BBa_C0061 misc_feature 680..704 /label=Help:Barcodes CDS 62..679 /label=BBa_C0061 misc_feature 44..704 /label=BBa_C0261 stem_loop 724..767 /label=stem_loop misc_feature 713..792 /label=BBa_B0010 terminator 713..792 /label=BBa_B0010 stem_loop 808..827 /label=T7 TE misc_feature 834..834 /label=stop polyA_site 828..841 /label=polya misc_feature 801..841 /label=BBa_B0012 terminator 801..841 /label=BBa_B0012 terminator 713..841 /label=BBa_B0015 misc_feature 44..841 /label=BBa_K082014 ORIGIN 1 tttatagcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta 61 gatgactata atgataaaaa aatcggattt tttggcaatt ccatcggagg agtataaagg 121 tattctaagt cttcgttatc aagtgtttaa gcaaagactt gagtgggact tagttgtaga 181 aaataacctt gaatcagatg agtatgataa ctcaaatgca gaatatattt atgcttgtga 241 tgatactgaa aatgtaagtg gatgctggcg tttattacct acaacaggtg attatatgct 301 gaaaagtgtt tttcctgaat tgcttggtca acagagtgct cccaaagatc ctaatatagt 361 cgaattaagt cgttttgctg taggtaaaaa tagctcaaag ataaataact ctgctagtga 421 aattacaatg aaactatttg aagctatata taaacacgct gttagtcaag gtattacaga 481 atatgtaaca gtaacatcaa cagcaataga gcgattttta aagcgtatta aagttccttg 541 tcatcgtatt ggagacaaag aaattcatgt attaggtgat actaaatcgg ttgtattgtc 601 tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat gctgcaaacg acgaaaacta 661 cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag agccaggcat 721 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 781 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 841 a //