LOCUS BBa_K176026 1942 bp DNA linear 22-Nov-2024 DEFINITION TetR repressible AHL->GFP Receiver (Very High LuxR): pCon 0.70->luxR+pLux/Tet->GFP ACCESSION BBa_K176026 VERSION BBa_K176026.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23101 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 62..63 /label=prefix misc_feature 235..235 /label=T misc_feature 553..553 /label=A CDS 62..811 /label=luxR misc_feature 62..817 /label=BBa_C0062 misc_feature 818..842 /label=Help:Barcodes CDS 62..817 /label=BBa_C0062 stem_loop 862..905 /label=stem_loop misc_feature 851..930 /label=BBa_B0010 terminator 851..930 /label=BBa_B0010 stem_loop 946..965 /label=T7 TE misc_feature 972..972 /label=stop polyA_site 966..979 /label=polya misc_feature 939..979 /label=BBa_B0012 terminator 939..979 /label=BBa_B0012 misc_feature 44..979 /label=BBa_I0462 misc_feature 1..979 /label=BBa_K176025 misc_feature 988..1040 /label=R0062 promoter 1007..1012 /label=-35 promoter 1029..1034 /label=-10 misc_feature 1041..1059 /label=tetR misc_feature 1040..1040 /label=+1 misc_feature 988..1007 /label=luxR/HSL promoter 988..1059 /label=BBa_K176000 misc_feature 1072..1075 /label=conserved RBS 1068..1079 /label=BBa_B0034 CDS 1086..1805 /label=GFP protein CDS 1086..1805 /label=BBa_E0040 stem_loop 1825..1868 /label=stem_loop misc_feature 1814..1893 /label=BBa_B0010 terminator 1814..1893 /label=BBa_B0010 stem_loop 1909..1928 /label=T7 TE misc_feature 1935..1935 /label=stop polyA_site 1929..1942 /label=polya misc_feature 1902..1942 /label=BBa_B0012 terminator 1902..1942 /label=BBa_B0012 misc_feature 988..1942 /label=BBa_K176002 ORIGIN 1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta 61 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag 121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata 181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga 241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc 301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa 361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac 421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca 481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt 541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa 601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc 661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa 721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac 781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc 841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 961 gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa 1021 tggtttgtta tagtcgaata tccctatcag tgatagagat actagagaaa gaggagaaat 1081 actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag 1141 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat 1201 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa 1261 cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga 1321 aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat 1381 ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc 1441 ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac 1501 acaaattgga atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga 1561 atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc gttcaactag 1621 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc 1681 attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg 1741 tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat 1801 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 1861 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 1921 ggtgggcctt tctgcgttta ta //