BBa_K176026
type: ComponentDefinition
persistent identity: BBa K176026
display id: BBa_K176026
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible AHL->GFP Receiver (Very High LuxR): pCon 0.70->luxR+pLux/Tet->GFP
created: 2009-07-15T11:00:00Z
modified: 2015-05-08T01:11:01Z
mutable provenance: K176025 K176002
owned by: james
owned by: myers
mutable description: AHL -> GFP TetR repressible
discontinued: false
dominant: false
experience: experience/Works
group u list: _282_
m user id: 0
owner id: 3908
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Input: AHL Output: GFP Design: pCon 0.70(J23101) generate LuxR pLux/Tet(K176000) generate GFP, activated by LuxR and AHL, repressed by TetR and no aTc
star: false
creator: Danqian Liu, Chao Li, Hao Jiang
type: DnaRegion
role: SO:0000804
role: partType/Signalling
component: BBa_K176002
component: BBa_K176025
sequence annotation: BBa_K176025
sequence annotation: BBa_K176002
sequence: https://synbiohub.org/public/igem/BBa_K176026_sequence/1

BBa_K176002
type: Component
persistent identity: component2283589
display id: component2283589
version: 1
top level: BBa_K176026
definition: BBa_K176002
access: public

BBa_K176025
type: Component
persistent identity: component2283571
display id: component2283571
version: 1
top level: BBa_K176026
definition: LuxR
access: public

BBa_K176025
type: SequenceAnnotation
persistent identity: annotation2283571
display id: annotation2283571
version: 1
top level: BBa_K176026
location: https://synbiohub.org/public/igem/BBa_K176026/annotation2283571/range2283571/1
component: BBa_K176025

https://synbiohub.org/public/igem/BBa_K176026/annotation2283571/range2283571/1
type: Range
persistent identity: range2283571
display id: range2283571
version: 1
top level: BBa_K176026
direction: direction/reverse
start: 1
end: 979
orientation: inline

BBa_K176002
type: SequenceAnnotation
persistent identity: annotation2283589
display id: annotation2283589
version: 1
top level: BBa_K176026
location: https://synbiohub.org/public/igem/BBa_K176026/annotation2283589/range2283589/1
component: BBa_K176002

https://synbiohub.org/public/igem/BBa_K176026/annotation2283589/range2283589/1
type: Range
persistent identity: range2283589
display id: range2283589
version: 1
top level: BBa_K176026
direction: direction/reverse
start: 988
end: 1942
orientation: inline

BBa_K176002
type: ComponentDefinition
persistent identity: BBa K176002
display id: BBa_K176002
version: 1
was generated by: iGEM to SBOL conversion
description: pLux/Tet(K176000)(LuxR+,TetR-)->RBS+GFP+T
created: 2009-06-13T11:00:00Z
modified: 2015-05-08T01:11:00Z
mutable provenance: K176000 I13504
owned by: james
owned by: myers
mutable description: Hybrid promoter Plux-tet + RBS + GFP + Terminator
discontinued: false
dominant: false
experience: experience/Works
group u list: _282_
m user id: 0
owner id: 3971
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Hybrid promoter Plux-tet->GFP
star: false
creator: Chao Li,Danqian Liu,Hao Jiang
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0012
component: BBa_B0034
component: BBa_K176000
component: BBa_B0010
component: BBa_E0040
sequence annotation: BBa_K176000
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K176002_sequence/1

BBa_B0012
type: Component
persistent identity: component2005359
display id: component2005359
version: 1
top level: BBa_K176002
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component2005354
display id: component2005354
version: 1
top level: BBa_K176002
definition: BBa_B0034
access: public

BBa_K176000
type: Component
persistent identity: component2005352
display id: component2005352
version: 1
top level: BBa_K176002
definition: pLux/Tet
access: public

BBa_B0010
type: Component
persistent identity: component2005357
display id: component2005357
version: 1
top level: BBa_K176002
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component2005356
display id: component2005356
version: 1
top level: BBa_K176002
definition: GFP
access: public

BBa_K176000
type: SequenceAnnotation
persistent identity: annotation2005352
display id: annotation2005352
version: 1
top level: BBa_K176002
location: https://synbiohub.org/public/igem/BBa_K176002/annotation2005352/range2005352/1
component: BBa_K176000

https://synbiohub.org/public/igem/BBa_K176002/annotation2005352/range2005352/1
type: Range
persistent identity: range2005352
display id: range2005352
version: 1
top level: BBa_K176002
direction: direction/reverse
start: 1
end: 72
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2005354
display id: annotation2005354
version: 1
top level: BBa_K176002
location: https://synbiohub.org/public/igem/BBa_K176002/annotation2005354/range2005354/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K176002/annotation2005354/range2005354/1
type: Range
persistent identity: range2005354
display id: range2005354
version: 1
top level: BBa_K176002
direction: direction/reverse
start: 81
end: 92
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2005356
display id: annotation2005356
version: 1
top level: BBa_K176002
location: https://synbiohub.org/public/igem/BBa_K176002/annotation2005356/range2005356/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K176002/annotation2005356/range2005356/1
type: Range
persistent identity: range2005356
display id: range2005356
version: 1
top level: BBa_K176002
direction: direction/reverse
start: 99
end: 818
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2005357
display id: annotation2005357
version: 1
top level: BBa_K176002
location: https://synbiohub.org/public/igem/BBa_K176002/annotation2005357/range2005357/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K176002/annotation2005357/range2005357/1
type: Range
persistent identity: range2005357
display id: range2005357
version: 1
top level: BBa_K176002
direction: direction/forward
start: 827
end: 906
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2005359
display id: annotation2005359
version: 1
top level: BBa_K176002
location: https://synbiohub.org/public/igem/BBa_K176002/annotation2005359/range2005359/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K176002/annotation2005359/range2005359/1
type: Range
persistent identity: range2005359
display id: range2005359
version: 1
top level: BBa_K176002
direction: direction/forward
start: 915
end: 955
orientation: inline

LuxR
type: ComponentDefinition
persistent identity: BBa K176025
display id: BBa_K176025
version: 1
was generated by: iGEM to SBOL conversion
description: pCon 0.70(J23101)->RBS+luxR+T
created: 2009-07-15T11:00:00Z
modified: 2015-05-08T01:11:01Z
mutable provenance: J23101 I0462
owned by: james
owned by: myers
mutable description: pCon 0.70(J23101)+luxR(I0462)
discontinued: false
dominant: false
experience: experience/Works
group u list: _282_
m user id: 0
owner id: 3908
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: pCon 0.70(J23101) generate LuxR
star: false
creator: Danqian Liu, Chao Li, Hao Jiang
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J23101
component: BBa_I0462
sequence annotation: BBa_J23101
sequence annotation: BBa_I0462
sequence: https://synbiohub.org/public/igem/BBa_K176025_sequence/1

BBa_J23101
type: Component
persistent identity: component2255337
display id: component2255337
version: 1
top level: LuxR
definition: BBa_J23101
access: public

BBa_I0462
type: Component
persistent identity: component2255352
display id: component2255352
version: 1
top level: LuxR
definition: LuxR
access: public

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2255337
display id: annotation2255337
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_K176025/annotation2255337/range2255337/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K176025/annotation2255337/range2255337/1
type: Range
persistent identity: range2255337
display id: range2255337
version: 1
top level: LuxR
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_I0462
type: SequenceAnnotation
persistent identity: annotation2255352
display id: annotation2255352
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_K176025/annotation2255352/range2255352/1
component: BBa_I0462

https://synbiohub.org/public/igem/BBa_K176025/annotation2255352/range2255352/1
type: Range
persistent identity: range2255352
display id: range2255352
version: 1
top level: LuxR
direction: direction/reverse
start: 44
end: 979
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

LuxR
type: ComponentDefinition
persistent identity: BBa I0462
display id: BBa_I0462
version: 1
was generated by: iGEM to SBOL conversion
description: luxR Protein Generator
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Produces LuxR protein which can sense 3OC6HSL in the media and activate transcription from R0062, the right hand Lux promoter
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_C0062
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I0462_sequence/1

BBa_B0034
type: Component
persistent identity: component943166
display id: component943166
version: 1
top level: LuxR
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component943189
display id: component943189
version: 1
top level: LuxR
definition: BBa_B0010
access: public

BBa_C0062
type: Component
persistent identity: component943181
display id: component943181
version: 1
top level: LuxR
definition: luxr
access: public

BBa_B0012
type: Component
persistent identity: component943199
display id: component943199
version: 1
top level: LuxR
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943166
display id: annotation943166
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943166/range943166/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0462/annotation943166/range943166/1
type: Range
persistent identity: range943166
display id: range943166
version: 1
top level: LuxR
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation943181
display id: annotation943181
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943181/range943181/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I0462/annotation943181/range943181/1
type: Range
persistent identity: range943181
display id: range943181
version: 1
top level: LuxR
direction: direction/forward
start: 19
end: 774
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943189
display id: annotation943189
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943189/range943189/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0462/annotation943189/range943189/1
type: Range
persistent identity: range943189
display id: range943189
version: 1
top level: LuxR
direction: direction/forward
start: 808
end: 887
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943199
display id: annotation943199
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943199/range943199/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0462/annotation943199/range943199/1
type: Range
persistent identity: range943199
display id: range943199
version: 1
top level: LuxR
direction: direction/forward
start: 896
end: 936
orientation: inline

BBa_J23101
type: ComponentDefinition
persistent identity: BBa J23101
display id: BBa_J23101
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23101_sequence/1

pLux/Tet
type: ComponentDefinition
persistent identity: BBa K176000
display id: BBa_K176000
version: 1
was generated by: iGEM to SBOL conversion
description: pLux/Tet Hybrid Promoter: (LuxR+,TetR-)->PoPS
created: 2009-05-05T11:00:00Z
modified: 2015-05-08T01:11:00Z
mutable provenance: Tet Operator sequence from BBa_R0040
owned by: james
owned by: myers
mutable description: sense TWO INPUTS, activation by AHL and repression by tetR
discontinued: false
dominant: false
experience: experience/Works
group u list: _282_
m user id: 0
owner id: 4010
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: How to arrange the two parts together?
star: false
creator: Danqian Liu, Chao Li, Hao Jiang
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: luxR/HSL
sequence annotation: R0062
sequence annotation: -35
sequence annotation: -10
sequence annotation: +1
sequence annotation: tetR
sequence: https://synbiohub.org/public/igem/BBa_K176000_sequence/1

luxR/HSL
type: SequenceAnnotation
persistent identity: annotation2002612
display id: annotation2002612
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002612/range2002612/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K176000/annotation2002612/range2002612/1
type: Range
persistent identity: range2002612
display id: range2002612
version: 1
top level: pLux/Tet
direction: direction/forward
start: 1
end: 20
orientation: inline

R0062
type: SequenceAnnotation
persistent identity: annotation2002607
display id: annotation2002607
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002607/range2002607/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K176000/annotation2002607/range2002607/1
type: Range
persistent identity: range2002607
display id: range2002607
version: 1
top level: pLux/Tet
direction: direction/forward
start: 1
end: 53
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2002608
display id: annotation2002608
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002608/range2002608/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K176000/annotation2002608/range2002608/1
type: Range
persistent identity: range2002608
display id: range2002608
version: 1
top level: pLux/Tet
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002609
display id: annotation2002609
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002609/range2002609/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K176000/annotation2002609/range2002609/1
type: Range
persistent identity: range2002609
display id: range2002609
version: 1
top level: pLux/Tet
direction: direction/forward
start: 42
end: 47
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation2002611
display id: annotation2002611
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002611/range2002611/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K176000/annotation2002611/range2002611/1
type: Range
persistent identity: range2002611
display id: range2002611
version: 1
top level: pLux/Tet
direction: direction/forward
start: 53
end: 53
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation2002610
display id: annotation2002610
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002610/range2002610/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K176000/annotation2002610/range2002610/1
type: Range
persistent identity: range2002610
display id: range2002610
version: 1
top level: pLux/Tet
direction: direction/forward
start: 54
end: 72
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

https://synbiohub.org/public/igem/BBa_K176026_sequence/1
type: Sequence
persistent identity: BBa K176026 sequence
display id: BBa_K176026_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176002_sequence/1
type: Sequence
persistent identity: BBa K176002 sequence
display id: BBa_K176002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176025_sequence/1
type: Sequence
persistent identity: BBa K176025 sequence
display id: BBa_K176025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0462_sequence/1
type: Sequence
persistent identity: BBa I0462 sequence
display id: BBa_I0462_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23101_sequence/1
type: Sequence
persistent identity: BBa J23101 sequence
display id: BBa_J23101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176000_sequence/1
type: Sequence
persistent identity: BBa K176000 sequence
display id: BBa_K176000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z