LOCUS       BBa_K176027              498 bp    DNA     linear       28-Sep-2024
DEFINITION  PoPS->RBS+lacZalpha-ccdB->PoPS
ACCESSION   BBa_K176027
VERSION     BBa_K176027.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..183
                     /label=a part of lacZalpha (I732006)
     misc_feature    19..21
                     /label=Start
     misc_feature    34..34
                     /label=177bp and a in J32026
     primer_bind     39..55
                     /label=M13 -20 primer
     primer_bind     59..82
                     /label=M13 -47 primer (K176059)
     primer_bind     59..75
                     /label=M13 -40 primer
     misc_feature    183..183
                     /label=g and 69bp in I732006
     misc_feature    190..191
                     /label=at in K145151
     misc_feature    190..495
                     /label=ccdB (K145151)
     misc_feature    273..273
                     /label=c in K145151
     misc_feature    493..498
                     /label=Stop
     primer_bind     206..233
                     /label=VRccdB primer (K176058)
     CDS             19..492
                     /label=lacZalpha-ccdB
     CDS             19..498
                     /label=BBa_K176003
ORIGIN
        1 aaagaggaga aatactagat gaccatgatt acggattcac tggccgtcgt tttacaacgt
       61 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      121 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      181 ctatacgtac ggcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt
      241 gtggatgtac agagtgatat tattgacacg ccggggcgac ggatggtgat ccccctggcc
      301 agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg
      361 gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg
      421 gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg
      481 ttctggggaa tataataa
//