##gff-version 3
##sequence-region BBa_K176027 1 498
BBa_K176027	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K176027	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176027	.	CDS	19	498	.	+	0	ID=BBa_K176003;Name=BBa_K176003
BBa_K176027	.	point_mutation	273	273	.	+	0	ID=annotation2008009;Name=c in K145151;Parent=BBa_K176003
BBa_K176027	.	primer_binding_site	206	233	.	+	0	ID=annotation2011971;Name=VRccdB primer (K176058);Parent=BBa_K176003
BBa_K176027	.	engineered_region	190	495	.	+	0	ID=annotation2008008;Name=ccdB (K145151);Parent=BBa_K176003
BBa_K176027	.	primer_binding_site	59	82	.	+	0	ID=annotation2008004;Name=M13 -47 primer (K176059);Parent=BBa_K176003
BBa_K176027	.	sequence_alteration	190	191	.	+	0	ID=annotation2008007;Name=at in K145151;Parent=BBa_K176003
BBa_K176027	.	engineered_region	19	183	.	+	0	ID=annotation2008000;Name=a part of lacZalpha (I732006);Parent=BBa_K176003
BBa_K176027	.	primer_binding_site	59	75	.	+	0	ID=annotation2008005;Name=M13 -40 primer;Parent=BBa_K176003
BBa_K176027	.	stop_codon	493	498	.	+	0	ID=annotation2008010;Name=Stop;Parent=BBa_K176003
BBa_K176027	.	point_mutation	183	183	.	+	0	ID=annotation2008006;Name=g and 69bp in I732006;Parent=BBa_K176003
BBa_K176027	.	sequence_alteration	34	34	.	+	0	ID=annotation2008002;Name=177bp and a in J32026;Parent=BBa_K176003
BBa_K176027	.	start_codon	19	21	.	+	0	ID=annotation2008001;Name=Start;Parent=BBa_K176003
BBa_K176027	.	CDS	19	492	.	+	0	ID=annotation2020665;Name=lacZalpha-ccdB;Parent=BBa_K176003
BBa_K176027	.	primer_binding_site	39	55	.	+	0	ID=annotation2008003;Name=M13 -20 primer;Parent=BBa_K176003
>BBa_K176027
aaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctg
gcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcg
cccttcccaacagttgcgcagcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtctg
tttgtggatgtacagagtgatattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctgt
cagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggc
cagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaac
ctgatgttctggggaatataataa