LOCUS BBa_K176029 2732 bp DNA linear 14-Nov-2024 DEFINITION Repressible AHL->GFP Receiver: PoPS->tetR+pCon 0.70->luxR+pLux/Tet- >GFP ACCESSION BBa_K176029 VERSION BBa_K176029.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..645 /label=tetR CDS 19..645 /label=BBa_I732080 stem_loop 665..708 /label=stem_loop misc_feature 654..733 /label=BBa_B0010 terminator 654..733 /label=BBa_B0010 stem_loop 749..768 /label=T7 TE misc_feature 775..775 /label=stop polyA_site 769..782 /label=polya misc_feature 742..782 /label=BBa_B0012 terminator 742..782 /label=BBa_B0012 misc_feature 1..782 /label=BBa_K176001 promoter 791..825 /label=BBa_J23101 misc_feature 838..841 /label=conserved RBS 834..845 /label=BBa_B0034 misc_feature 852..853 /label=prefix misc_feature 1025..1025 /label=T misc_feature 1343..1343 /label=A CDS 852..1601 /label=luxR misc_feature 852..1607 /label=BBa_C0062 misc_feature 1608..1632 /label=Help:Barcodes CDS 852..1607 /label=BBa_C0062 stem_loop 1652..1695 /label=stem_loop misc_feature 1641..1720 /label=BBa_B0010 terminator 1641..1720 /label=BBa_B0010 stem_loop 1736..1755 /label=T7 TE misc_feature 1762..1762 /label=stop polyA_site 1756..1769 /label=polya misc_feature 1729..1769 /label=BBa_B0012 terminator 1729..1769 /label=BBa_B0012 misc_feature 834..1769 /label=BBa_I0462 misc_feature 791..1769 /label=BBa_K176025 misc_feature 1778..1830 /label=R0062 promoter 1797..1802 /label=-35 promoter 1819..1824 /label=-10 misc_feature 1831..1849 /label=tetR misc_feature 1830..1830 /label=+1 misc_feature 1778..1797 /label=luxR/HSL promoter 1778..1849 /label=BBa_K176000 misc_feature 1862..1865 /label=conserved RBS 1858..1869 /label=BBa_B0034 CDS 1876..2595 /label=GFP protein CDS 1876..2595 /label=BBa_E0040 stem_loop 2615..2658 /label=stem_loop misc_feature 2604..2683 /label=BBa_B0010 terminator 2604..2683 /label=BBa_B0010 stem_loop 2699..2718 /label=T7 TE misc_feature 2725..2725 /label=stop polyA_site 2719..2732 /label=polya misc_feature 2692..2732 /label=BBa_B0012 terminator 2692..2732 /label=BBa_B0012 misc_feature 1778..2732 /label=BBa_K176002 misc_feature 791..2732 /label=BBa_K176026 ORIGIN 1 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta 61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta 121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc 181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc 241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc 301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa 361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc 421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct 481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc 541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata 601 tgcggattag aaaaacaact taaatgtgaa agtgggtcct aataatacta gagccaggca 661 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 721 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 781 tatactagag tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg 841 agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta 901 aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac 961 attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt 1021 caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa 1081 aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat tggaatatat 1141 ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag 1201 gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta 1261 gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga 1321 acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata 1381 ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag 1441 gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc 1501 atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag 1561 caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct 1621 agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 1681 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 1741 accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta 1801 cgcaagaaaa tggtttgtta tagtcgaata tccctatcag tgatagagat actagagaaa 1861 gaggagaaat actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt 1921 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt 1981 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt 2041 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca 2101 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa 2161 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa 2221 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac 2281 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac 2341 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc 2401 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta 2461 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga 2521 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa 2581 ctatacaaat aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 2641 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 2701 ctcaccttcg ggtgggcctt tctgcgttta ta //