##gff-version 3
##sequence-region BBa_K176030 1 2790
BBa_K176030	.	engineered_region	1	840	.	+	0	ID=BBa_K176006;Name=BBa_K176006
BBa_K176030	.	engineered_region	1	703	.	+	0	ID=BBa_S0101;Name=BBa_S0101;Parent=BBa_K176006
BBa_K176030	.	CDS	19	678	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S0101
BBa_K176030	.	engineered_tag	679	703	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K176030	.	tag	639	672	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K176030	.	CDS	22	638	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K176030	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0101
BBa_K176030	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176030	.	terminator	712	840	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K176006
BBa_K176030	.	terminator	712	791	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K176030	.	stem_loop	723	766	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176030	.	engineered_region	712	791	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176030	.	terminator	800	840	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K176030	.	polyA_site	827	840	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176030	.	stop_codon	833	833	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176030	.	engineered_region	800	840	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176030	.	stem_loop	807	826	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176030	.	engineered_region	849	2790	.	+	0	ID=BBa_K176026;Name=BBa_K176026
BBa_K176030	.	engineered_region	849	1827	.	+	0	ID=BBa_K176025;Name=BBa_K176025;Parent=BBa_K176026
BBa_K176030	.	engineered_region	892	1827	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K176025
BBa_K176030	.	terminator	1699	1778	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K176030	.	stem_loop	1710	1753	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176030	.	engineered_region	1699	1778	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176030	.	ribosome_entry_site	892	903	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K176030	.	conserved_region	896	899	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176030	.	terminator	1787	1827	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K176030	.	polyA_site	1814	1827	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176030	.	stop_codon	1820	1820	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176030	.	engineered_region	1787	1827	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176030	.	stem_loop	1794	1813	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176030	.	CDS	910	1665	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K176030	.	engineered_region	910	1665	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K176030	.	CDS	910	1659	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K176030	.	engineered_region	910	911	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K176030	.	point_mutation	1083	1083	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K176030	.	engineered_tag	1666	1690	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K176030	.	point_mutation	1401	1401	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K176030	.	promoter	849	883	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K176025
BBa_K176030	.	engineered_region	1836	2790	.	+	0	ID=BBa_K176002;Name=BBa_K176002;Parent=BBa_K176026
BBa_K176030	.	terminator	2662	2741	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K176002
BBa_K176030	.	stem_loop	2673	2716	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K176030	.	engineered_region	2662	2741	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K176030	.	ribosome_entry_site	1916	1927	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K176002
BBa_K176030	.	conserved_region	1920	1923	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K176030	.	promoter	1836	1907	.	+	0	ID=BBa_K176000;Name=BBa_K176000;Parent=BBa_K176002
BBa_K176030	.	promoter	1855	1860	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K176030	.	promoter	1877	1882	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K176030	.	non_covalent_binding_site	1889	1907	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K176030	.	start_codon	1888	1888	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K176030	.	non_covalent_binding_site	1836	1855	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K176030	.	engineered_region	1836	1888	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K176030	.	terminator	2750	2790	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K176002
BBa_K176030	.	polyA_site	2777	2790	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K176030	.	stop_codon	2783	2783	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K176030	.	engineered_region	2750	2790	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K176030	.	stem_loop	2757	2776	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K176030	.	CDS	1934	2653	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K176002
BBa_K176030	.	CDS	1934	2653	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_K176030
aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcg
gaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaa
taagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaa
agctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtac
atttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttc
actagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaa
gtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatc
accaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaag
tgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtccta
ggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataatt
aataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaat
attatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatg
gaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaatt
aattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtctta
tcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaa
ctatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaa
ataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaa
aaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaa
actcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaat
taataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatc
agtgatagagatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaatt
cttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaa
aacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatgg
tgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgta
caggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttg
ttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataa
ctcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaa
gatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaacc
attacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaac
agctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggc
tcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacct
tcgggtgggcctttctgcgtttata