LOCUS       BBa_K176031             1849 bp    DNA     linear       14-Nov-2024
DEFINITION  Repressible AHL->PoPS Receiver: PoPS->tetR+pCon 
            0.70->luxR+pLux/Tet->PoPS
ACCESSION   BBa_K176031
VERSION     BBa_K176031.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..645
                     /label=tetR
     CDS             19..645
                     /label=BBa_I732080
     stem_loop       665..708
                     /label=stem_loop
     misc_feature    654..733
                     /label=BBa_B0010
     terminator      654..733
                     /label=BBa_B0010
     stem_loop       749..768
                     /label=T7 TE
     misc_feature    775..775
                     /label=stop
     polyA_site      769..782
                     /label=polya
     misc_feature    742..782
                     /label=BBa_B0012
     terminator      742..782
                     /label=BBa_B0012
     misc_feature    1..782
                     /label=BBa_K176001
     promoter        791..825
                     /label=BBa_J23101
     misc_feature    838..841
                     /label=conserved
     RBS             834..845
                     /label=BBa_B0034
     misc_feature    852..853
                     /label=prefix
     misc_feature    1025..1025
                     /label=T
     misc_feature    1343..1343
                     /label=A
     CDS             852..1601
                     /label=luxR
     misc_feature    852..1607
                     /label=BBa_C0062
     misc_feature    1608..1632
                     /label=Help:Barcodes
     CDS             852..1607
                     /label=BBa_C0062
     stem_loop       1652..1695
                     /label=stem_loop
     misc_feature    1641..1720
                     /label=BBa_B0010
     terminator      1641..1720
                     /label=BBa_B0010
     stem_loop       1736..1755
                     /label=T7 TE
     misc_feature    1762..1762
                     /label=stop
     polyA_site      1756..1769
                     /label=polya
     misc_feature    1729..1769
                     /label=BBa_B0012
     terminator      1729..1769
                     /label=BBa_B0012
     misc_feature    834..1769
                     /label=BBa_I0462
     misc_feature    791..1769
                     /label=BBa_K176025
     misc_feature    1778..1830
                     /label=R0062
     promoter        1797..1802
                     /label=-35
     promoter        1819..1824
                     /label=-10
     misc_feature    1831..1849
                     /label=tetR
     misc_feature    1830..1830
                     /label=+1
     misc_feature    1778..1797
                     /label=luxR/HSL
     promoter        1778..1849
                     /label=BBa_K176000
     misc_feature    791..1849
                     /label=BBa_K176028
ORIGIN
        1 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
       61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
      121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
      181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
      241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
      301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
      361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
      421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
      481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
      541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
      601 tgcggattag aaaaacaact taaatgtgaa agtgggtcct aataatacta gagccaggca
      661 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      721 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      781 tatactagag tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg
      841 agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta
      901 aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac
      961 attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt
     1021 caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa
     1081 aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat tggaatatat
     1141 ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag
     1201 gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta
     1261 gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga
     1321 acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata
     1381 ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag
     1441 gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc
     1501 atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag
     1561 caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct
     1621 agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1681 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1741 accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta
     1801 cgcaagaaaa tggtttgtta tagtcgaata tccctatcag tgatagaga
//